FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W85097951> ?p ?o ?g. }

• W85097951 endingPage "28" @default.
• W85097951 startingPage "219" @default.
• W85097951 abstract "Calu-3 cells secrete HCO(3)(-) in response to cAMP agonists but can be stimulated to secrete Cl(-) with K(+) channel activating agonists. Microelectrode and impedance analysis experiments were performed to obtain a better understanding of the conductances and driving forces involved in these different modes of anion secretion in Calu-3 cells. Microelectrode studies revealed apical and basolateral membrane depolarizations upon the addition of forskolin (V(ap) -52 mV vs. -21 mV; V(bl) -60 mV vs. -44 mV) that paralleled the hyperpolarization of the mucosal negative transepithelial voltage (V(T) -8 mV vs. -23 mV). These changes were accompanied by a decrease in the apical membrane fractional resistance (F(Rap)) from approximately 0.50 to 0.08, consistent with the activation of an apical membrane conductance. The subsequent addition of 1-ethyl-2-benzimidazolinone (1-EBIO), a K(+) channel activator, hyperpolarized V(ap) to -27 mV, V(bl) to -60 mV and V(T) to -33 mV. Impedance analysis revealed the apical membrane resistance (R(ap)) of the forskolin-stimulated cells was less than 20 ohm cm(2), indeed in most monolayers R(ap) fell to less than 5 ohm cm(2). The impedance derived estimate of the basolateral membrane resistance (R(bl)) was approximately 170 ohm cm(2) in forskolin treated cells and fell to 50 ohm cm(2) with the addition of 1-EBIO. Using these values for the R(bl) and the F(Rap) value of 0.08 yields a R(ap) of approximately 14 ohm cm(2) in the presence of forskolin and 4 ohm cm(2) in the presence of forskolin plus 1-EBIO. Thus, by two independent methods, forskolin-stimulated Calu-3 cells are seen to have a very high apical membrane conductance of 50 to 200 mS/cm(2). Therefore, we would assert that even at one-tenth the anion selectivity for Cl(-), this high conductance could support the conductive exit of HCO(3)(-) across the apical membrane. We further propose that this high apical membrane conductance serves to clamp the apical membrane potential near the equilibrium potential for Cl(-) and thereby provides the driving force for HCO(3)(-) secretion in forskolin-stimulated Calu-3 cells. The hyperpolarization of V(ap) and V(bl) caused by 1-EBIO provides a driving force for Cl(-) exit across the apical membrane, inhibits the influx of HCO(3)(-) on the Na(+):HCO(3)(-) cotransporter across the basolateral membrane, activates the basolateral membrane Na(+):K:2Cl(-) cotransporter and thereby provides the switch from HCO(3)(-) secretion to Cl(-) secretion." @default.
• W85097951 created "2016-06-24" @default.
• W85097951 creator A5012552638 @default.
• W85097951 creator A5019057851 @default.
• W85097951 creator A5026190252 @default.
• W85097951 creator A5033410717 @default.
• W85097951 date "2001-07-01" @default.
• W85097951 modified "2023-09-23" @default.
• W85097951 title "Microelectrode and impedance analysis of anion secretion in Calu-3 cells." @default.
• W85097951 cites W1973728279 @default.
• W85097951 cites W1975215165 @default.
• W85097951 cites W2026751820 @default.
• W85097951 cites W2045419222 @default.
• W85097951 cites W2064359124 @default.
• W85097951 cites W2089976468 @default.
• W85097951 cites W2102730957 @default.
• W85097951 cites W2129557473 @default.
• W85097951 cites W2148028731 @default.
• W85097951 cites W2148324275 @default.
• W85097951 cites W2149085480 @default.
• W85097951 cites W2259743896 @default.
• W85097951 cites W2287882369 @default.
• W85097951 cites W2336230469 @default.
• W85097951 cites W2399022058 @default.
• W85097951 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11875263" @default.
• W85097951 hasPublicationYear "2001" @default.
• W85097951 type Work @default.
• W85097951 sameAs 85097951 @default.
• W85097951 citedByCount "7" @default.
• W85097951 countsByYear W850979512012 @default.
• W85097951 countsByYear W850979512018 @default.
• W85097951 countsByYear W850979512020 @default.
• W85097951 countsByYear W850979512021 @default.
• W85097951 crossrefType "journal-article" @default.
• W85097951 hasAuthorship W85097951A5012552638 @default.
• W85097951 hasAuthorship W85097951A5019057851 @default.
• W85097951 hasAuthorship W85097951A5026190252 @default.
• W85097951 hasAuthorship W85097951A5033410717 @default.
• W85097951 hasConcept C111670793 @default.
• W85097951 hasConcept C113196181 @default.
• W85097951 hasConcept C121332964 @default.
• W85097951 hasConcept C121932024 @default.
• W85097951 hasConcept C12554922 @default.
• W85097951 hasConcept C126322002 @default.
• W85097951 hasConcept C131453863 @default.
• W85097951 hasConcept C134018914 @default.
• W85097951 hasConcept C147789679 @default.
• W85097951 hasConcept C17525397 @default.
• W85097951 hasConcept C181911157 @default.
• W85097951 hasConcept C185592680 @default.
• W85097951 hasConcept C202751555 @default.
• W85097951 hasConcept C26873012 @default.
• W85097951 hasConcept C2779276759 @default.
• W85097951 hasConcept C3018436504 @default.
• W85097951 hasConcept C32211213 @default.
• W85097951 hasConcept C41625074 @default.
• W85097951 hasConcept C43617362 @default.
• W85097951 hasConcept C49039625 @default.
• W85097951 hasConcept C55493867 @default.
• W85097951 hasConcept C62520636 @default.
• W85097951 hasConcept C66974803 @default.
• W85097951 hasConcept C71240020 @default.
• W85097951 hasConcept C71924100 @default.
• W85097951 hasConcept C86803240 @default.
• W85097951 hasConceptScore W85097951C111670793 @default.
• W85097951 hasConceptScore W85097951C113196181 @default.
• W85097951 hasConceptScore W85097951C121332964 @default.
• W85097951 hasConceptScore W85097951C121932024 @default.
• W85097951 hasConceptScore W85097951C12554922 @default.
• W85097951 hasConceptScore W85097951C126322002 @default.
• W85097951 hasConceptScore W85097951C131453863 @default.
• W85097951 hasConceptScore W85097951C134018914 @default.
• W85097951 hasConceptScore W85097951C147789679 @default.
• W85097951 hasConceptScore W85097951C17525397 @default.
• W85097951 hasConceptScore W85097951C181911157 @default.
• W85097951 hasConceptScore W85097951C185592680 @default.
• W85097951 hasConceptScore W85097951C202751555 @default.
• W85097951 hasConceptScore W85097951C26873012 @default.
• W85097951 hasConceptScore W85097951C2779276759 @default.
• W85097951 hasConceptScore W85097951C3018436504 @default.
• W85097951 hasConceptScore W85097951C32211213 @default.
• W85097951 hasConceptScore W85097951C41625074 @default.
• W85097951 hasConceptScore W85097951C43617362 @default.
• W85097951 hasConceptScore W85097951C49039625 @default.
• W85097951 hasConceptScore W85097951C55493867 @default.
• W85097951 hasConceptScore W85097951C62520636 @default.
• W85097951 hasConceptScore W85097951C66974803 @default.
• W85097951 hasConceptScore W85097951C71240020 @default.
• W85097951 hasConceptScore W85097951C71924100 @default.
• W85097951 hasConceptScore W85097951C86803240 @default.
• W85097951 hasIssue "4 Suppl" @default.
• W85097951 hasLocation W850979511 @default.
• W85097951 hasOpenAccess W85097951 @default.
• W85097951 hasPrimaryLocation W850979511 @default.
• W85097951 hasRelatedWork W2001720486 @default.
• W85097951 hasRelatedWork W2069051539 @default.
• W85097951 hasRelatedWork W2076876209 @default.
• W85097951 hasRelatedWork W2121145715 @default.

• next