Matches in SemOpenAlex for { <https://semopenalex.org/work/W85138467> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W85138467 abstract "Firefly luciferase is a hydrophobic enzyme that which catalyses the oxidation of luciferin by molecular oxygen and requires the enzymatic cofactors ATP and magnesium ions. This enzymatic reaction is accompanied by intense luminescence. Luciferase is generally considered a membrane-associated protein. Most of the current extensive knowledge of luciferase enzymology has been been carried out in aqueous systems in which the lifetime of bioluminescence following the injection of ATP is on the order of seconds. Therefore, the kinetics of its reaction in membrane-mimetic systems such as the colloidal reverse micelle system would be expected to be quite different. Following the initial report of Belyaeva et. al.1, we decided to investigate the effect of pH on the spectral and kinetic properties of the luciferase bioluminescence reaction in colloidal reverse micelles2 formed from the interaction of the nonionic surfactant Brij 96, water and cyclohexane. Previous aqueous luciferase investigations have shown that as the pH is decreased from 8.0, the bioluminescence emission maxima shifts from 562 nm, the same as observed in the in vivo bioluminescence, to > 600 nm, with a corresponding decrease in the bioluminescence quantum efficiency." @default.
- W85138467 created "2016-06-24" @default.
- W85138467 creator A5015800982 @default.
- W85138467 creator A5076357158 @default.
- W85138467 creator A5083338178 @default.
- W85138467 date "1988-01-01" @default.
- W85138467 modified "2023-09-23" @default.
- W85138467 title "Luciferase Reverse Micellar Bioluminescence: Kinetics and Emission Spectra" @default.
- W85138467 cites W4211049950 @default.
- W85138467 doi "https://doi.org/10.1007/978-1-4684-5568-7_35" @default.
- W85138467 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3250480" @default.
- W85138467 hasPublicationYear "1988" @default.
- W85138467 type Work @default.
- W85138467 sameAs 85138467 @default.
- W85138467 citedByCount "0" @default.
- W85138467 crossrefType "book-chapter" @default.
- W85138467 hasAuthorship W85138467A5015800982 @default.
- W85138467 hasAuthorship W85138467A5076357158 @default.
- W85138467 hasAuthorship W85138467A5083338178 @default.
- W85138467 hasConcept C104317684 @default.
- W85138467 hasConcept C111335760 @default.
- W85138467 hasConcept C11268172 @default.
- W85138467 hasConcept C121332964 @default.
- W85138467 hasConcept C12554922 @default.
- W85138467 hasConcept C148898269 @default.
- W85138467 hasConcept C17189327 @default.
- W85138467 hasConcept C178790620 @default.
- W85138467 hasConcept C184651966 @default.
- W85138467 hasConcept C185592680 @default.
- W85138467 hasConcept C192562407 @default.
- W85138467 hasConcept C2781309008 @default.
- W85138467 hasConcept C2985930086 @default.
- W85138467 hasConcept C49040817 @default.
- W85138467 hasConcept C5176359 @default.
- W85138467 hasConcept C54009773 @default.
- W85138467 hasConcept C55493867 @default.
- W85138467 hasConcept C62520636 @default.
- W85138467 hasConcept C75473681 @default.
- W85138467 hasConcept C86803240 @default.
- W85138467 hasConceptScore W85138467C104317684 @default.
- W85138467 hasConceptScore W85138467C111335760 @default.
- W85138467 hasConceptScore W85138467C11268172 @default.
- W85138467 hasConceptScore W85138467C121332964 @default.
- W85138467 hasConceptScore W85138467C12554922 @default.
- W85138467 hasConceptScore W85138467C148898269 @default.
- W85138467 hasConceptScore W85138467C17189327 @default.
- W85138467 hasConceptScore W85138467C178790620 @default.
- W85138467 hasConceptScore W85138467C184651966 @default.
- W85138467 hasConceptScore W85138467C185592680 @default.
- W85138467 hasConceptScore W85138467C192562407 @default.
- W85138467 hasConceptScore W85138467C2781309008 @default.
- W85138467 hasConceptScore W85138467C2985930086 @default.
- W85138467 hasConceptScore W85138467C49040817 @default.
- W85138467 hasConceptScore W85138467C5176359 @default.
- W85138467 hasConceptScore W85138467C54009773 @default.
- W85138467 hasConceptScore W85138467C55493867 @default.
- W85138467 hasConceptScore W85138467C62520636 @default.
- W85138467 hasConceptScore W85138467C75473681 @default.
- W85138467 hasConceptScore W85138467C86803240 @default.
- W85138467 hasLocation W851384671 @default.
- W85138467 hasLocation W851384672 @default.
- W85138467 hasOpenAccess W85138467 @default.
- W85138467 hasPrimaryLocation W851384671 @default.
- W85138467 hasRelatedWork W1980223867 @default.
- W85138467 hasRelatedWork W1980800538 @default.
- W85138467 hasRelatedWork W2023973953 @default.
- W85138467 hasRelatedWork W2053886780 @default.
- W85138467 hasRelatedWork W2078957528 @default.
- W85138467 hasRelatedWork W2081654454 @default.
- W85138467 hasRelatedWork W2119275063 @default.
- W85138467 hasRelatedWork W2803934923 @default.
- W85138467 hasRelatedWork W4210932807 @default.
- W85138467 hasRelatedWork W53587174 @default.
- W85138467 isParatext "false" @default.
- W85138467 isRetracted "false" @default.
- W85138467 magId "85138467" @default.
- W85138467 workType "book-chapter" @default.