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- W851956647 startingPage "826" @default.
- W851956647 abstract "为了解高迁移率族蛋白B族（high mobility group protein B,HMGB）基因调控植物响应低温、高盐和干旱等外源胁迫的表达调控方式,本文克隆了拟南芥AtHMGB前5个家族成员的启动子区域（PAtHMGB1,PAtHMGB2,PAtHMGB3,PAtHMGB4和PAtHMGB5）.运用基因重组技术将其分别替换表达载体上35S启动子区域获得重组表达载体,利用农杆菌介导法侵染烟草获得稳定表达的转基因烟草.运用实时定量PCR检测上述5种启动子的转基因烟草,观察在外源胁迫（低温、高盐和干旱）处理前后gusA基因的表达差异,同时检测转基因烟草种子在不同外源胁迫条件下的萌发状况.检测结果证实,在低温胁迫下,PAtHMGB2,PAtHMGB3和PAtHMGB4正调控gusA基因的表达,而在干旱或盐胁迫下,gusA基因的表达被PAtHMGB2和PAtHMGB3负调控.种子萌发结果表明,在干旱胁迫下,PAtHMGB2调控下的转基因烟草比野生型烟草萌发及生长迟缓;在低温胁迫下,PAtHMGB2调控的转基因烟草长势明显强于野生型.本研究克隆了拟南芥AtHMGB家族前5个成员启动子,分析其生物学功能发现,PAtHMGB2在响应低温和干旱胁迫方面效果尤为显著." @default.
- W851956647 created "2016-06-24" @default.
- W851956647 creator A5029589129 @default.
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- W851956647 date "2012-09-26" @default.
- W851956647 modified "2023-09-24" @default.
- W851956647 title "拟南芥高迁移率族蛋白B（AtHMGB）族基因启动子的转录激活功能分析" @default.
- W851956647 hasPublicationYear "2012" @default.
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