FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W85304853> ?p ?o ?g. }

• W85304853 endingPage "50" @default.
• W85304853 startingPage "327" @default.
• W85304853 abstract "The effects of 5 mM potassium cyanide (KCN) on creatine phosphokinase (CPK) release and cellular morphology were studied. Rat hearts were perfused with substrate-deficient media gassed with O2 or N2 (O2 medium, N2 medium) at 37 C, and effluent was collected for creatine phosphokinase analysis. Tissue fixation was with glutaraldehyde for light and electron microscopy. Experiments included the following: a) continuous perfusion with O2- or N2-medium in the presence of KCN; b) 45 or 60 minutes of perfusion with N2-medium followed by O2-medium for 15 or 180 minutes, respectively; c) 45 minutes of perfusion with N2-medium with KCN added 15 minutes before reoxygenation with O2-medium plus KCN; (4) 60 minutes of N2-medium plus KCN followed by O2-medium plus KCN for 180 minutes; d) as a control for irreversible injury, 21 minutes of perfusion with calcium-free O2-medium followed by 2.5 mM calcium-O2-medium (calcium paradox). The following results were seen: a) Initial CPK release occurred about 30 minutes later from hearts perfused with O2-medium plus KCN than from hearts perfused with N2-medium plus KCN. b) Upon reoxygenation after either 45 or 60 minutes of anoxia, hearts had a sudden peak of oxygen-induced CPK release. Most irreversibly injured cells were massively swollen and had sarcolemmal defects and contraction bands. Reversibly injured cells in the same hearts resembled normal myocardium. A previously unrecognized third population of cells is described. These cells were characterized by contraction bands but were not swollen, had intact sarcolemma, and contained both normal and damaged mitochondria with intramatrical calcium accumulation granules. It could not be determined if these cells were reversibly injured or in an early stage of irreversible injury. c) KCN added 15 minutes before reoxygenation of hearts after 45 minutes of anoxia inhibited the sudden peak of oxygen-induced CPK release but not a slow sustained release. Small to moderate numbers of cells in these hearts contained contraction bands. d) After 60 minutes, KCN completely inhibited both oxygen-induced CPK release and contraction band formation. e) Addition of calcium to calcium-free hearts caused both massive CPK release and contraction band formation. It is concluded that: the beginning of CPK release from oxygenated KCN-inhibited hearts requires about 30 minutes longer than from anoxic hearts; KCN can inhibit both oxygen-induced CPK release and contraction bands in irreversibly injured rat myocardial cells; sudden contracture of myocardial cells as occurs in the calcium paradox can result in massive CPK release; contraction bands occur in nonswollen cells, hence contraction bands can occur independently of massive cell swelling or membrane rupture. It is postulated that there may be two stages of irreversible myocardial injury; a) loss of control of contraction and b) progressive loss of mitochondrial and membrane integrity." @default.
• W85304853 created "2016-06-24" @default.
• W85304853 creator A5006032475 @default.
• W85304853 creator A5037807620 @default.
• W85304853 creator A5075747630 @default.
• W85304853 date "1976-08-01" @default.
• W85304853 modified "2023-09-23" @default.
• W85304853 title "Oxygen-induced enzyme release after irreversible myocardial injury. Effects of cyanide in perfused rat hearts." @default.
• W85304853 cites W1427695666 @default.
• W85304853 cites W1459172789 @default.
• W85304853 cites W1528492966 @default.
• W85304853 cites W1556330567 @default.
• W85304853 cites W1647735571 @default.
• W85304853 cites W1735059796 @default.
• W85304853 cites W1834461289 @default.
• W85304853 cites W1879092365 @default.
• W85304853 cites W1965317569 @default.
• W85304853 cites W1973879310 @default.
• W85304853 cites W2023111733 @default.
• W85304853 cites W2032497553 @default.
• W85304853 cites W2037981435 @default.
• W85304853 cites W2046419819 @default.
• W85304853 cites W2048394915 @default.
• W85304853 cites W2050443626 @default.
• W85304853 cites W2051909997 @default.
• W85304853 cites W2053873939 @default.
• W85304853 cites W2059047888 @default.
• W85304853 cites W2071757034 @default.
• W85304853 cites W2080473810 @default.
• W85304853 cites W2082943134 @default.
• W85304853 cites W2115486837 @default.
• W85304853 cites W2136819544 @default.
• W85304853 cites W2144537953 @default.
• W85304853 cites W2144708655 @default.
• W85304853 cites W2246105844 @default.
• W85304853 cites W2248087544 @default.
• W85304853 cites W2397109807 @default.
• W85304853 cites W2410906853 @default.
• W85304853 cites W241116528 @default.
• W85304853 cites W2412299056 @default.
• W85304853 cites W2414885354 @default.
• W85304853 cites W2418361993 @default.
• W85304853 cites W2418640361 @default.
• W85304853 cites W2423542819 @default.
• W85304853 cites W2471464769 @default.
• W85304853 cites W60748611 @default.
• W85304853 cites W621768210 @default.
• W85304853 cites W1946684358 @default.
• W85304853 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2032457" @default.
• W85304853 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/941982" @default.
• W85304853 hasPublicationYear "1976" @default.
• W85304853 type Work @default.
• W85304853 sameAs 85304853 @default.
• W85304853 citedByCount "33" @default.
• W85304853 countsByYear W853048532018 @default.
• W85304853 countsByYear W853048532020 @default.
• W85304853 crossrefType "journal-article" @default.
• W85304853 hasAuthorship W85304853A5006032475 @default.
• W85304853 hasAuthorship W85304853A5037807620 @default.
• W85304853 hasAuthorship W85304853A5075747630 @default.
• W85304853 hasConcept C126322002 @default.
• W85304853 hasConcept C146957229 @default.
• W85304853 hasConcept C163415756 @default.
• W85304853 hasConcept C178790620 @default.
• W85304853 hasConcept C179104552 @default.
• W85304853 hasConcept C185592680 @default.
• W85304853 hasConcept C199096232 @default.
• W85304853 hasConcept C2776424019 @default.
• W85304853 hasConcept C2778455166 @default.
• W85304853 hasConcept C2778626300 @default.
• W85304853 hasConcept C2779133510 @default.
• W85304853 hasConcept C2908647359 @default.
• W85304853 hasConcept C2986317502 @default.
• W85304853 hasConcept C36880943 @default.
• W85304853 hasConcept C41625074 @default.
• W85304853 hasConcept C519063684 @default.
• W85304853 hasConcept C540031477 @default.
• W85304853 hasConcept C55493867 @default.
• W85304853 hasConcept C71924100 @default.
• W85304853 hasConcept C99454951 @default.
• W85304853 hasConceptScore W85304853C126322002 @default.
• W85304853 hasConceptScore W85304853C146957229 @default.
• W85304853 hasConceptScore W85304853C163415756 @default.
• W85304853 hasConceptScore W85304853C178790620 @default.
• W85304853 hasConceptScore W85304853C179104552 @default.
• W85304853 hasConceptScore W85304853C185592680 @default.
• W85304853 hasConceptScore W85304853C199096232 @default.
• W85304853 hasConceptScore W85304853C2776424019 @default.
• W85304853 hasConceptScore W85304853C2778455166 @default.
• W85304853 hasConceptScore W85304853C2778626300 @default.
• W85304853 hasConceptScore W85304853C2779133510 @default.
• W85304853 hasConceptScore W85304853C2908647359 @default.
• W85304853 hasConceptScore W85304853C2986317502 @default.
• W85304853 hasConceptScore W85304853C36880943 @default.
• W85304853 hasConceptScore W85304853C41625074 @default.
• W85304853 hasConceptScore W85304853C519063684 @default.
• W85304853 hasConceptScore W85304853C540031477 @default.
• W85304853 hasConceptScore W85304853C55493867 @default.

• next