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- W853137797 abstract "[Objective] The aim was to clone Syntaxin genes in Limonium sinense Kuntze. [Method] Limonium sinense Kuntze leaves were used as materials and total RNA was extracted and transcribed reversely. Nested primers were designed as per EST sequences at gene 5’ region of Syntaxin, and cDNA obtained through reverse reaction was taken as the template. Sequences of Syntaxin gene at 3’ region were achieved through two rounds of PCR amplifications. [Result] DNA fragments (1 096 bp) were obtained. For LsSyntaxin, open reading frame (ORF) was 816 bp and the encoded amino acids were 271. The relative molecular weight of Syntaxin was 30 254.3 Da and isoelectric point in theory was 5.55. [Conclusion] Syntaxin genes from Limonium sinense Kuntze were cloned. The research laid foundation for study of Syntaxin gene function in Limonium sinense Kuntze and salt-secreted process." @default.
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- W853137797 date "2012-01-01" @default.
- W853137797 modified "2023-09-27" @default.
- W853137797 title "Cloning of Syntaxin gene in Limonium sinense Kuntzet." @default.
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