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- W853592053 abstract "CONDAS, L. A. Z. Phenotypic and genotypic characterization and thermorressitance to boiling of strains of the Nocardia spp. genus isolated from domestic animals and humans. 2011, 104p, dissertation (Masters degree) – Faculdade de Medicina Veterinaria e Zootecnia, Campus de Botucatu, Universidade Estadual Paulista “Julio de Mesquita Filho”. Botucatu, SP. Nocardia is an ubiquitous microorganism, environmental, related to supurative pyogranulomatous of difficult treatment in humans and domestic animals. The phenotypic and genotypic characterization, antimicrobial susceptibility profile and thermorresistance to boiling of 95 strains of Nocardia spp., from both domestic animals and humans, were investigated. In production animals the most common affection was the bovine mastitis, identified in 71 strains (74,73%). Among the small animal, most of the isolates were from dogs, a total of 12 strains (12,63%), especially from cutaneous manifestations. Seven isolates were obtained from humans, with emphasis for cases with cutaneous and neurological signs. The molecular characterization and the phylogenetic analysis made through PCR of the 16S rRNA resulted in the identification: Nocardia nova (68,42%), Nocardia farcinica (13,68%), Nocardia cyriacigeorgica (4,58%), Nocardia puris (3,67%), Nocardia veterana (3,67%), Nocardia africana (2,10%), Nocardia asiatica (0,91%) and Nocardia arthritidis (0,91%). The occurrence of multi-resistance to three or more and five or more antimicrobials was observed in 42,88% and 14,28%of the studied strains, respectively. The comparison between the susceptibility profile through disc diffusion and the minimum inhibitory concentration showed a weak correlation, except for amikacin. In the evaluation of thermorresistance to boiling 84,21% of the strains were re-isolated after reaching 100°C in milk, 50,52% after boiling for one minute at 100oC and 2,10% after boiling for 5 minutes. This study showed high similarity between Nocardia sp. strains isolated from domestic animals and humans, increased multi-resistance to some of the most used antimicrobials in the therapy of nocardiosis in animals and humans, thermorresistance to the" @default.
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- W853592053 date "2011-03-22" @default.
- W853592053 modified "2023-09-23" @default.
- W853592053 title "Caracterização fenotípica, genotípica e termorressistência à fervura em linhagens de Nocardia spp isoladas de animais domésticos e humanos" @default.
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- W853592053 hasPublicationYear "2011" @default.
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