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• W853695314 abstract "B virus diagnosis presents a challenge largely complicated by the asymptomatic infection of rhesus macaques, and extremely pathogenic fatal infections in humans. Humoral detection of antibodies is generally performed using whole virus antigen for which preparation requires strict biosafety measures and specialized BSL-4 facilities. As an alternative to utilizing B virus antigen, we describe the production of a truncated form of B virus envelope glycoprotein D, gD 287, in a baculovirus expression system, and evaluate its diagnostic potential as an antigen in recombinant ELISA. After purification and characterization, gD 287 was tested using 22 negative and 72 positive macaque sera samples previously classified using the traditional method. We find that sensitivity and specificity of the recombinant ELISA are dependent on antibody titer of tested serum and gD 287 shows good to excellent predictive potential for identification of positive sera with titers higher than 500. INDEX WORDS: Serodiagnosis, Herpes virus, Glycoprotein D, Recombinant, ELISA, Rhesus macaque, cercopithecine. PRODUCTION OF B VIRUS GLYCOPROTEIN D AND EVALUATION OF ITS DIAGNOSTIC POTENTIAL" @default.
• W853695314 created "2016-06-24" @default.
• W853695314 creator A5026578075 @default.
• W853695314 date "2008-01-01" @default.
• W853695314 modified "2023-09-26" @default.
• W853695314 title "Production of B Virus Glycoprotein D and Evaluation of its Diagnostic Potential" @default.
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