FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W854621591> ?p ?o ?g. }

• W854621591 endingPage "110" @default.
• W854621591 startingPage "100" @default.
• W854621591 abstract "Full kinetic models provide insight into enzyme mechanism and kinetics and also support bioconversion process design and feasibility assessment. Previously we have established automated microwell methods for rapid data collection and hybrid kinetic modelling techniques for quantification of kinetic constants. In this work these methods are applied to explore the substrate selectivity and kinetics of monoamine oxidase, MAO-N-D5, from Aspergillus niger. In particular we examine the MAO-N-D5 variant Ile246Met/Asn336Ser/Met348Lys/Thr384Asn to allow the oxidation of secondary amines Initial screening showed that MAO-N-D5 enabled the selective oxidation of secondary amines in 8 and 9 carbon rings, as well as primary ethyl and propyl amines attached to secondary amines of indolines and pyrrolidines. Subsequently we developed a first kinetic model for the MAO-N-D5 enzyme based on the ping-pong bi-bi mechanism (similar to that for the human MAO-A enzyme). The full set of kinetic parameters were then established for three MAO-N-D5 substrates namely; 3-azabicyclo[3,3,0]octane, 1-(2 amino ethyl) pyrrolidine and 3-(2,3-dihydro-1H-indole-1-yl)propan-1-amine. The models for each amine substrate showed excellent agreement with experimentally determined progress curves over a range of operating conditions. They indicated that in each case amine inhibition was the main determinant of overall reaction rate rather than oxygen or imine (product) inhibition. From the perspective of larger scale bioconversion process design, the models indicated the need for fed-batch addition of the amine substrate and to increase the dissolved oxygen levels in order to maximize bioconversion process productivity." @default.
• W854621591 created "2016-06-24" @default.
• W854621591 creator A5006309408 @default.
• W854621591 creator A5009546172 @default.
• W854621591 creator A5021098447 @default.
• W854621591 creator A5028055831 @default.
• W854621591 creator A5039222257 @default.
• W854621591 creator A5043396605 @default.
• W854621591 date "2015-10-01" @default.
• W854621591 modified "2023-10-18" @default.
• W854621591 title "High throughput screening of monoamine oxidase (MAO-N-D5) substrate selectivity and rapid kinetic model generation" @default.
• W854621591 cites W1963531926 @default.
• W854621591 cites W1976236461 @default.
• W854621591 cites W1994897786 @default.
• W854621591 cites W1999250213 @default.
• W854621591 cites W2018134213 @default.
• W854621591 cites W2019552004 @default.
• W854621591 cites W2021486592 @default.
• W854621591 cites W2026048837 @default.
• W854621591 cites W2037943939 @default.
• W854621591 cites W2051879033 @default.
• W854621591 cites W2053834100 @default.
• W854621591 cites W2055725578 @default.
• W854621591 cites W2067452122 @default.
• W854621591 cites W2069163132 @default.
• W854621591 cites W2073530095 @default.
• W854621591 cites W2075392544 @default.
• W854621591 cites W2084071209 @default.
• W854621591 cites W2085358206 @default.
• W854621591 cites W2088286639 @default.
• W854621591 cites W2098474598 @default.
• W854621591 cites W2098840696 @default.
• W854621591 cites W2101591208 @default.
• W854621591 cites W2104832758 @default.
• W854621591 cites W2117305247 @default.
• W854621591 cites W2126559862 @default.
• W854621591 cites W2130131457 @default.
• W854621591 cites W2136624548 @default.
• W854621591 cites W2161420671 @default.
• W854621591 cites W2164461070 @default.
• W854621591 cites W2168078928 @default.
• W854621591 cites W4293247451 @default.
• W854621591 doi "https://doi.org/10.1016/j.molcatb.2015.07.001" @default.
• W854621591 hasPublicationYear "2015" @default.
• W854621591 type Work @default.
• W854621591 sameAs 854621591 @default.
• W854621591 citedByCount "8" @default.
• W854621591 countsByYear W8546215912016 @default.
• W854621591 countsByYear W8546215912017 @default.
• W854621591 countsByYear W8546215912021 @default.
• W854621591 countsByYear W8546215912022 @default.
• W854621591 countsByYear W8546215912023 @default.
• W854621591 crossrefType "journal-article" @default.
• W854621591 hasAuthorship W854621591A5006309408 @default.
• W854621591 hasAuthorship W854621591A5009546172 @default.
• W854621591 hasAuthorship W854621591A5021098447 @default.
• W854621591 hasAuthorship W854621591A5028055831 @default.
• W854621591 hasAuthorship W854621591A5039222257 @default.
• W854621591 hasAuthorship W854621591A5043396605 @default.
• W854621591 hasBestOaLocation W8546215911 @default.
• W854621591 hasConcept C114461151 @default.
• W854621591 hasConcept C118792377 @default.
• W854621591 hasConcept C121332964 @default.
• W854621591 hasConcept C135889238 @default.
• W854621591 hasConcept C157764524 @default.
• W854621591 hasConcept C161790260 @default.
• W854621591 hasConcept C181199279 @default.
• W854621591 hasConcept C185592680 @default.
• W854621591 hasConcept C18903297 @default.
• W854621591 hasConcept C21951064 @default.
• W854621591 hasConcept C2776024655 @default.
• W854621591 hasConcept C2777289219 @default.
• W854621591 hasConcept C2994592520 @default.
• W854621591 hasConcept C41008148 @default.
• W854621591 hasConcept C51323132 @default.
• W854621591 hasConcept C55493867 @default.
• W854621591 hasConcept C555944384 @default.
• W854621591 hasConcept C62520636 @default.
• W854621591 hasConcept C76155785 @default.
• W854621591 hasConcept C86803240 @default.
• W854621591 hasConceptScore W854621591C114461151 @default.
• W854621591 hasConceptScore W854621591C118792377 @default.
• W854621591 hasConceptScore W854621591C121332964 @default.
• W854621591 hasConceptScore W854621591C135889238 @default.
• W854621591 hasConceptScore W854621591C157764524 @default.
• W854621591 hasConceptScore W854621591C161790260 @default.
• W854621591 hasConceptScore W854621591C181199279 @default.
• W854621591 hasConceptScore W854621591C185592680 @default.
• W854621591 hasConceptScore W854621591C18903297 @default.
• W854621591 hasConceptScore W854621591C21951064 @default.
• W854621591 hasConceptScore W854621591C2776024655 @default.
• W854621591 hasConceptScore W854621591C2777289219 @default.
• W854621591 hasConceptScore W854621591C2994592520 @default.
• W854621591 hasConceptScore W854621591C41008148 @default.
• W854621591 hasConceptScore W854621591C51323132 @default.
• W854621591 hasConceptScore W854621591C55493867 @default.
• W854621591 hasConceptScore W854621591C555944384 @default.
• W854621591 hasConceptScore W854621591C62520636 @default.

• next