Matches in SemOpenAlex for { <https://semopenalex.org/work/W854697795> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W854697795 endingPage "321" @default.
- W854697795 startingPage "314" @default.
- W854697795 abstract "We have cloned the Bacillus cellulyticus K-12 avicelase (Avi, E.C.3.2.1.4) gene (ace A) In E. coli. This was accompanied by using the vector PT7T3U 19 and Hind W -Hind m libraries of Bacillus cellulyticus K-12 chromosomal inserts created in 5.cofi. The Libraries were screened for the expression of avicelase by monitoring the immunoreaction of the anti-avicelase (immunoscreening). Positive clones (Ac-3, Ac-5, and Ac-7) contained the identical 3.5kb Hind III fragment as determined by restriction mapping and Southern hybridization, and expressed avicelase efficiently and constituvely using its own promoter in the heterologous host. From the immunoblotting analysis, a polypeptide which showed a CMCase activity with an Mr of 54000 was detected." @default.
- W854697795 created "2016-06-24" @default.
- W854697795 creator A5000386730 @default.
- W854697795 creator A5074483970 @default.
- W854697795 date "1993-01-01" @default.
- W854697795 modified "2023-09-24" @default.
- W854697795 title "Bacillus cellulyticus K-12 Crystalline Cellulose-Degrading Avicelase Gene and Expression in Eschterichia coli" @default.
- W854697795 hasPublicationYear "1993" @default.
- W854697795 type Work @default.
- W854697795 sameAs 854697795 @default.
- W854697795 citedByCount "0" @default.
- W854697795 crossrefType "journal-article" @default.
- W854697795 hasAuthorship W854697795A5000386730 @default.
- W854697795 hasAuthorship W854697795A5074483970 @default.
- W854697795 hasConcept C104317684 @default.
- W854697795 hasConcept C108305142 @default.
- W854697795 hasConcept C153911025 @default.
- W854697795 hasConcept C2776295094 @default.
- W854697795 hasConcept C40767141 @default.
- W854697795 hasConcept C523546767 @default.
- W854697795 hasConcept C54355233 @default.
- W854697795 hasConcept C86803240 @default.
- W854697795 hasConcept C89423630 @default.
- W854697795 hasConceptScore W854697795C104317684 @default.
- W854697795 hasConceptScore W854697795C108305142 @default.
- W854697795 hasConceptScore W854697795C153911025 @default.
- W854697795 hasConceptScore W854697795C2776295094 @default.
- W854697795 hasConceptScore W854697795C40767141 @default.
- W854697795 hasConceptScore W854697795C523546767 @default.
- W854697795 hasConceptScore W854697795C54355233 @default.
- W854697795 hasConceptScore W854697795C86803240 @default.
- W854697795 hasConceptScore W854697795C89423630 @default.
- W854697795 hasIssue "4" @default.
- W854697795 hasLocation W8546977951 @default.
- W854697795 hasOpenAccess W854697795 @default.
- W854697795 hasPrimaryLocation W8546977951 @default.
- W854697795 hasRelatedWork W111727231 @default.
- W854697795 hasRelatedWork W123977162 @default.
- W854697795 hasRelatedWork W1484266445 @default.
- W854697795 hasRelatedWork W1984196627 @default.
- W854697795 hasRelatedWork W1989022856 @default.
- W854697795 hasRelatedWork W2004634964 @default.
- W854697795 hasRelatedWork W2005943302 @default.
- W854697795 hasRelatedWork W2019593322 @default.
- W854697795 hasRelatedWork W2094035349 @default.
- W854697795 hasRelatedWork W2347885442 @default.
- W854697795 hasRelatedWork W2368748569 @default.
- W854697795 hasRelatedWork W2369823662 @default.
- W854697795 hasRelatedWork W2370634359 @default.
- W854697795 hasRelatedWork W2371441812 @default.
- W854697795 hasRelatedWork W2380894555 @default.
- W854697795 hasRelatedWork W2383730821 @default.
- W854697795 hasRelatedWork W2386371604 @default.
- W854697795 hasRelatedWork W2393020191 @default.
- W854697795 hasRelatedWork W2397412438 @default.
- W854697795 hasRelatedWork W405484546 @default.
- W854697795 hasVolume "6" @default.
- W854697795 isParatext "false" @default.
- W854697795 isRetracted "false" @default.
- W854697795 magId "854697795" @default.
- W854697795 workType "article" @default.