Matches in SemOpenAlex for { <https://semopenalex.org/work/W855601409> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W855601409 endingPage "642" @default.
- W855601409 startingPage "633" @default.
- W855601409 abstract "1. ETEC F41 균주로부터 분리한 섬모항원의 분자량은 29.5 kDa으로 나타났으며, western blot을 통하여 섬모항원임을 확인하였다. 2. 분리한 섬모항원의 농도를 50 <TEX>${mu}g$</TEX>/<TEX>$mell$</TEX>, 200 <TEX>${mu}g$</TEX>/<TEX>$mell$</TEX>, 600 <TEX>${mu}g$</TEX>/<TEX>$mell$</TEX>로 조정 후 산란계에 접종하였다. 이 후 ELISA법을 이용하여 난황의 항체역가를 측정한 결과 최고치가 320,000(antigen 50<TEX>${mu}g$</TEX>/<TEX>$mell$</TEX>), 450,000(antigen 200<TEX>${mu}g$</TEX>/<TEX>$mell$</TEX>), 320,000(anti- gen 600<TEX>${mu}g$</TEX>/<TEX>$mell$</TEX>)으로 나타났다. 3. F41난황항체와 K88, K99, 987P 섬모항원과의 교차반응을 ELISA법을 이용하여 조사해본 결과 난황항체를 30,000배 희석 시 교차반응이 없었다. 4. 실험실조건하에서 난황항체의 항원결합능력을 조사한 결과, 동결건조한 WSF을 2<TEX>${sim}$</TEX>4 mg/<TEX>$mell$</TEX> 첨가 시 균체의 농도가 <TEX>$10^9$</TEX> CFU/<TEX>$mell$</TEX>에서 <TEX>$10^5$</TEX> CFU/<TEX>$mell$</TEX>로 급격하게 감소하였다. Enteric colibacillosis has economically become an important disease of young animals as a result of increasing intensification of farrowing management. The objective of this experiment is to isolate fimbrial antigen from enterotoxigenic E. coli F41, to develop specific polyclonal IgY which can effectively neutralize or reduce the proliferation of pathogens in feed or living animal system, and to apply IgY technologies to animal industry. The results obtained were as follows: The molecular weight of the purified F41 antigen was 29,500 dalton on sodium dodecyl sulfate-polyacrylamide gels. Fimbrial antigen was confirmed by the western blot method. It was observed that after immunization the level of serum antibody titer of laying hen was shown in two weeks and gradually increased. The antibody titer in egg yolk appeared two weeks after it was shown in serum antibody. The titers of egg yolk antibody were gradually increased to the maximum level of 320,000 (antigen 50<TEX>${mu}g$</TEX>/<TEX>$mell$</TEX>), 450,000 (antigen 200<TEX>${mu}g$</TEX>/<TEX>$mell$</TEX>), and 320,000 (antigen 600<TEX>${mu}g$</TEX>/<TEX>$mell$</TEX>). According to the results of specificity test by ELISA, the anti-F41 antibodies from chicken serum and egg yolk reacted only with ETEC F41 antigen. There was no cross reaction with other ETEC strains (K88, K99, and 987P). In vitro condition, as a result of antigen binding ability of yolk antibodies, bacterial concentration was rapidly decreased to <TEX>$10^5$</TEX> CFU/<TEX>$mell$</TEX> from <TEX>$10^9$</TEX> CFU/<TEX>$mell$</TEX> when 2<TEX>${sim}$</TEX>4 mg/<TEX>$mell$</TEX> of freeze dried WSF (water soluble fraction) was added." @default.
- W855601409 created "2016-06-24" @default.
- W855601409 date "2002-10-31" @default.
- W855601409 modified "2023-10-18" @default.
- W855601409 title "Production of a Specific Yolk Antibody against Enterotoxigenic E. coli F41 Fimbrial Antigen" @default.
- W855601409 cites W1984199318 @default.
- W855601409 cites W2100837269 @default.
- W855601409 doi "https://doi.org/10.5187/jast.2002.44.5.633" @default.
- W855601409 hasPublicationYear "2002" @default.
- W855601409 type Work @default.
- W855601409 sameAs 855601409 @default.
- W855601409 citedByCount "0" @default.
- W855601409 crossrefType "journal-article" @default.
- W855601409 hasBestOaLocation W8556014091 @default.
- W855601409 hasConcept C104317684 @default.
- W855601409 hasConcept C147483822 @default.
- W855601409 hasConcept C153911025 @default.
- W855601409 hasConcept C159654299 @default.
- W855601409 hasConcept C185592680 @default.
- W855601409 hasConcept C203014093 @default.
- W855601409 hasConcept C22889606 @default.
- W855601409 hasConcept C2776415932 @default.
- W855601409 hasConcept C2777871010 @default.
- W855601409 hasConcept C2781321622 @default.
- W855601409 hasConcept C31903555 @default.
- W855601409 hasConcept C32611913 @default.
- W855601409 hasConcept C547475151 @default.
- W855601409 hasConcept C55493867 @default.
- W855601409 hasConcept C66999527 @default.
- W855601409 hasConcept C86803240 @default.
- W855601409 hasConcept C89423630 @default.
- W855601409 hasConcept C956191 @default.
- W855601409 hasConceptScore W855601409C104317684 @default.
- W855601409 hasConceptScore W855601409C147483822 @default.
- W855601409 hasConceptScore W855601409C153911025 @default.
- W855601409 hasConceptScore W855601409C159654299 @default.
- W855601409 hasConceptScore W855601409C185592680 @default.
- W855601409 hasConceptScore W855601409C203014093 @default.
- W855601409 hasConceptScore W855601409C22889606 @default.
- W855601409 hasConceptScore W855601409C2776415932 @default.
- W855601409 hasConceptScore W855601409C2777871010 @default.
- W855601409 hasConceptScore W855601409C2781321622 @default.
- W855601409 hasConceptScore W855601409C31903555 @default.
- W855601409 hasConceptScore W855601409C32611913 @default.
- W855601409 hasConceptScore W855601409C547475151 @default.
- W855601409 hasConceptScore W855601409C55493867 @default.
- W855601409 hasConceptScore W855601409C66999527 @default.
- W855601409 hasConceptScore W855601409C86803240 @default.
- W855601409 hasConceptScore W855601409C89423630 @default.
- W855601409 hasConceptScore W855601409C956191 @default.
- W855601409 hasIssue "5" @default.
- W855601409 hasLocation W8556014091 @default.
- W855601409 hasOpenAccess W855601409 @default.
- W855601409 hasPrimaryLocation W8556014091 @default.
- W855601409 hasRelatedWork W1829426772 @default.
- W855601409 hasRelatedWork W2354476437 @default.
- W855601409 hasRelatedWork W2357994263 @default.
- W855601409 hasRelatedWork W2360587303 @default.
- W855601409 hasRelatedWork W2371433017 @default.
- W855601409 hasRelatedWork W2376129311 @default.
- W855601409 hasRelatedWork W2381991529 @default.
- W855601409 hasRelatedWork W3028649718 @default.
- W855601409 hasRelatedWork W8052553 @default.
- W855601409 hasRelatedWork W855601409 @default.
- W855601409 hasVolume "44" @default.
- W855601409 isParatext "false" @default.
- W855601409 isRetracted "false" @default.
- W855601409 magId "855601409" @default.
- W855601409 workType "article" @default.