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- W85603600 abstract "Research focused on noninvasive techniques are essential for the conservation and management of wildlife in Ecuador. This study tested the methods SDS / CAI, CTAB / CAI and 2CTAB/CAI to extract genomic DNA Andean fox (Lycalopex culpaeus) from fecal samples collected in the Cayambe-Coca National Park. The results visualized on agarose gels showed a positive extraction for the three methods. DNA quantification and purity assessment by measuring its absorbance at 260/280 nm showed that the best method is the third (2CTAB/CAI) with average values of 51.16 ng/ul of DNA purity and 1.65. Once extracted DNA was amplified by PCR a 200 bp fragment of mitochondrial cytochrome b gene. The technique used is relatively inexpensive compared with extraction kits and opens up the possibility of several molecular ecology studies in the Andean fox in Ecuador." @default.
- W85603600 created "2016-06-24" @default.
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- W85603600 date "2012-09-24" @default.
- W85603600 modified "2023-09-23" @default.
- W85603600 title "Estandarización de un protocolo para la extracción de ADN de muestras fecales de lobo de páramo (Lycalopex culpaeus)" @default.
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