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• W85682461 abstract "Article1 April 1985free access Cloning of the extra sex combs gene of Drosophila and its identification by P-element-mediated gene transfer Erich Frei Erich Frei Department of Cell Biology, Biocenter of the University, CH-4056 Basel, Switzerland Search for more papers by this author Stefan Baumgartner Stefan Baumgartner Department of Cell Biology, Biocenter of the University, CH-4056 Basel, Switzerland Search for more papers by this author Jan-Erik Edström Jan-Erik Edström European Molecular Biology Laboratory, D-6900 Heidelberg, FRG Search for more papers by this author Markus Noll Markus Noll Department of Cell Biology, Biocenter of the University, CH-4056 Basel, Switzerland Search for more papers by this author Erich Frei Erich Frei Department of Cell Biology, Biocenter of the University, CH-4056 Basel, Switzerland Search for more papers by this author Stefan Baumgartner Stefan Baumgartner Department of Cell Biology, Biocenter of the University, CH-4056 Basel, Switzerland Search for more papers by this author Jan-Erik Edström Jan-Erik Edström European Molecular Biology Laboratory, D-6900 Heidelberg, FRG Search for more papers by this author Markus Noll Markus Noll Department of Cell Biology, Biocenter of the University, CH-4056 Basel, Switzerland Search for more papers by this author Author Information Erich Frei1, Stefan Baumgartner1, Jan-Erik Edström2 and Markus Noll1 1Department of Cell Biology, Biocenter of the University, CH-4056 Basel, Switzerland 2European Molecular Biology Laboratory, D-6900 Heidelberg, FRG The EMBO Journal (1985)4:979-987https://doi.org/10.1002/j.1460-2075.1985.tb03727.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info A large region containing the extra sex combs (esc) gene of Drosophila has been cloned by microdissection from polytene chromosomes and chromosomal walking. Within this DNA, the segment comprising the esc gene has been narrowed down in several steps. First, a deletion of the esc+ gene, Df(2L)esc10, defined a region of 380 kb. Then, a duplication carrying the esc+ gene, Dp(2;2)GYL, and a deletion not eliminating esc+, Df(2L)prd1.7.20, further reduced this region to 160 kb. Finally, because esc transcripts are expected to be present in follicles but absent in adult males, mapping of follicle and adult male transcripts limited the esc region to ˜10 kb. Rigorous proof that this DNA contained the esc+ gene was obtained by P-element-mediated transformation. We conclude that the 12 kb of DNA used for transformation include the esc+ gene as well as all flanking sequences required for its proper regulation in the female germ line. Previous ArticleNext Article Volume 4Issue 41 April 1985In this issue RelatedDetailsLoading ..." @default.
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• W85682461 title "Cloning of the <i>extra sex combs</i> gene of <i>Drosophila</i> and its identification by P-element-mediated gene transfer" @default.
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