Matches in SemOpenAlex for { <https://semopenalex.org/work/W85707285> ?p ?o ?g. }
- W85707285 abstract "One aim of computational protein design is to introduce novel enzyme activity into proteins of known structure by predicting mutations that stabilize transition states. Previously, we showed that it is possible to introduce triose phosphate isomerase activity into the ribose-binding protein of Escherichia coli by constructing 17 mutations in the first two layers of residues that surround the wild-type ligand-binding site. Here, we report that these mutations can be transplanted into a homologous ribose-binding protein, isolated from the hyperthermophilic bacterium Thermoanaerobacter tengcongensis, with retention of catalytic activity, substrate affinity, and reaction pH dependence. The observed 10(5)-10(6)-fold rate enhancement corresponds to 70% of the maximally known transition-state binding energy. The wild-type sequences in these two homologues are almost perfectly conserved in the vicinity of their ribose-binding sites, but diverge significantly at increasing distance from these sites. The results demonstrate that the computationally designed mutations are sufficient to encode the observed enzyme activity, that all the observed activity is encoded locally within the layer of residues directly in contact with the substrate and that, in this case, at least 70% of transition state stabilization energy can be achieved using straightforward considerations of stereochemical complementarity between enzyme and reactants." @default.
- W85707285 created "2016-06-24" @default.
- W85707285 creator A5020353852 @default.
- W85707285 creator A5055422020 @default.
- W85707285 creator A5074445091 @default.
- W85707285 date "2007-02-01" @default.
- W85707285 modified "2023-10-16" @default.
- W85707285 title "RETRACTED: Local Encoding of Computationally Designed Enzyme Activity" @default.
- W85707285 cites W1504164119 @default.
- W85707285 cites W1506579504 @default.
- W85707285 cites W1509956720 @default.
- W85707285 cites W1512790523 @default.
- W85707285 cites W1550727253 @default.
- W85707285 cites W1593975599 @default.
- W85707285 cites W1903489927 @default.
- W85707285 cites W1965205049 @default.
- W85707285 cites W1967779980 @default.
- W85707285 cites W1971099088 @default.
- W85707285 cites W1972784441 @default.
- W85707285 cites W1974579447 @default.
- W85707285 cites W1980568487 @default.
- W85707285 cites W1982792504 @default.
- W85707285 cites W1985676676 @default.
- W85707285 cites W1988251333 @default.
- W85707285 cites W1989052605 @default.
- W85707285 cites W1994593429 @default.
- W85707285 cites W2005167964 @default.
- W85707285 cites W2008517042 @default.
- W85707285 cites W2010421631 @default.
- W85707285 cites W2013263481 @default.
- W85707285 cites W2025088751 @default.
- W85707285 cites W2026019482 @default.
- W85707285 cites W2043502580 @default.
- W85707285 cites W2046002103 @default.
- W85707285 cites W2054130096 @default.
- W85707285 cites W2054534830 @default.
- W85707285 cites W2075157068 @default.
- W85707285 cites W2075598295 @default.
- W85707285 cites W2078382601 @default.
- W85707285 cites W2079193378 @default.
- W85707285 cites W2079346455 @default.
- W85707285 cites W2083144176 @default.
- W85707285 cites W2083997681 @default.
- W85707285 cites W2088329806 @default.
- W85707285 cites W2089467794 @default.
- W85707285 cites W2091664813 @default.
- W85707285 cites W2096921126 @default.
- W85707285 cites W2101043122 @default.
- W85707285 cites W2103622571 @default.
- W85707285 cites W2105722865 @default.
- W85707285 cites W2118768703 @default.
- W85707285 cites W2119586900 @default.
- W85707285 cites W2128635872 @default.
- W85707285 cites W2138817605 @default.
- W85707285 cites W2139982366 @default.
- W85707285 cites W2147850116 @default.
- W85707285 cites W2157271776 @default.
- W85707285 cites W2160294964 @default.
- W85707285 cites W2168393617 @default.
- W85707285 cites W2609912642 @default.
- W85707285 doi "https://doi.org/10.1016/j.jmb.2006.12.002" @default.
- W85707285 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2963085" @default.
- W85707285 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17196220" @default.
- W85707285 hasPublicationYear "2007" @default.
- W85707285 type Work @default.
- W85707285 sameAs 85707285 @default.
- W85707285 citedByCount "11" @default.
- W85707285 countsByYear W857072852016 @default.
- W85707285 crossrefType "journal-article" @default.
- W85707285 hasAuthorship W85707285A5020353852 @default.
- W85707285 hasAuthorship W85707285A5055422020 @default.
- W85707285 hasAuthorship W85707285A5074445091 @default.
- W85707285 hasBestOaLocation W857072852 @default.
- W85707285 hasConcept C104317684 @default.
- W85707285 hasConcept C143065580 @default.
- W85707285 hasConcept C181199279 @default.
- W85707285 hasConcept C185592680 @default.
- W85707285 hasConcept C55493867 @default.
- W85707285 hasConcept C86803240 @default.
- W85707285 hasConceptScore W85707285C104317684 @default.
- W85707285 hasConceptScore W85707285C143065580 @default.
- W85707285 hasConceptScore W85707285C181199279 @default.
- W85707285 hasConceptScore W85707285C185592680 @default.
- W85707285 hasConceptScore W85707285C55493867 @default.
- W85707285 hasConceptScore W85707285C86803240 @default.
- W85707285 hasLocation W857072851 @default.
- W85707285 hasLocation W857072852 @default.
- W85707285 hasLocation W857072853 @default.
- W85707285 hasLocation W857072854 @default.
- W85707285 hasOpenAccess W85707285 @default.
- W85707285 hasPrimaryLocation W857072851 @default.
- W85707285 hasRelatedWork W1971026909 @default.
- W85707285 hasRelatedWork W1974873606 @default.
- W85707285 hasRelatedWork W1977750999 @default.
- W85707285 hasRelatedWork W1997430881 @default.
- W85707285 hasRelatedWork W2013141503 @default.
- W85707285 hasRelatedWork W2041372545 @default.
- W85707285 hasRelatedWork W2072129035 @default.
- W85707285 hasRelatedWork W2074542695 @default.
- W85707285 hasRelatedWork W2097387866 @default.