Matches in SemOpenAlex for { <https://semopenalex.org/work/W857119568> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W857119568 abstract "Ischaemic stroke is a major cause of mortality and chronic disability for which there is no effective treatment. The subventricular zone (SVZ) is an adult neurogenic niche which mediates limited endogenous repair following stroke. To harness this phenomenon for therapy, it is important to understand how the SVZ niche is altered in stroke, and the processes that recruit neural precursors to the site of injury, which becomes a de facto neurogenic niche. Galectin-3 (Gal-3) is a β-galactoside binding protein involved in cellular adhesion, inflammation and tumour metastasis. Gal-3 is specifically expressed in the SVZ and maintains neuroblast migration to the olfactory bulb, although its role in post-stroke neurogenesis is not well-understood. Therefore, this project aimed to (1) characterise the cytoarchitecture of the SVZ in response to stroke, and (2) examine the role of Gal-3 in stroke outcome and tissue remodelling, and test the hypothesis that Gal-3 is required for neuroblast ectopic migration into the ischaemic striatum. Using the intraluminal filament model of middle cerebral artery occlusion (MCAO) in mice, and whole mounts of the lateral ventricular wall, significant SVZ reactive astrocytosis and increased vascular branching were observed, thereby disrupting the neuroblast migratory scaffold. Stroke increased SVZ cell proliferation without increase in cell death. Post-stroke ependymal cells were enlarged and non-proliferative, and assumed a reactive astroglial phenotype, expressing de novo high levels of glial fibrillary acidic protein. This was associated with focal planar cell polarity misalignment, and turbulent and decreased rate of cerebrospinal fluid flow. These findings demonstrate significant changes in multiple SVZ cell types which are positioned to influence post-stroke neurogenesis and regulation of the neural stem cell niche Gal-3 was up-regulated in the ischaemic brain and ipsilateral SVZ. To elucidate the role of Gal-3 after stroke, MCAO was performed in wildtype and Gal-3 null (Gal-3 -/- ) mice, and parameters of stroke outcome and post-stroke neurogenesis compared. The deletion of Gal-3 did not affect infarct volumes or neurological outcomes, although neuroblast migration into the ischaemic striatum was increased in Gal-3 -/- brains. Gal-3 -/- mice failed to mount an angiogenic response in the ischaemic striatum, and this was associated with lower levels of vascular endothelial growth factor (VEGF) and increased anti-angiogenic protein levels. Loss of Gal-3 further disrupted the pro-proliferative neural-vascular interaction at the basement membrane. The current data indicate that Gal-3 is a pleiotropic molecule which has distinct roles in both the SVZ and the post-stroke striatum as niches of adult neurogenesis." @default.
- W857119568 created "2016-06-24" @default.
- W857119568 creator A5009484336 @default.
- W857119568 date "2011-01-01" @default.
- W857119568 modified "2023-09-27" @default.
- W857119568 title "The adult neural stem cell niche in ischaemic stroke" @default.
- W857119568 hasPublicationYear "2011" @default.
- W857119568 type Work @default.
- W857119568 sameAs 857119568 @default.
- W857119568 citedByCount "0" @default.
- W857119568 crossrefType "dissertation" @default.
- W857119568 hasAuthorship W857119568A5009484336 @default.
- W857119568 hasConcept C127413603 @default.
- W857119568 hasConcept C136834591 @default.
- W857119568 hasConcept C142724271 @default.
- W857119568 hasConcept C169760540 @default.
- W857119568 hasConcept C202474031 @default.
- W857119568 hasConcept C204232928 @default.
- W857119568 hasConcept C2777542384 @default.
- W857119568 hasConcept C2779965356 @default.
- W857119568 hasConcept C2780495277 @default.
- W857119568 hasConcept C2780496858 @default.
- W857119568 hasConcept C2780645631 @default.
- W857119568 hasConcept C28328180 @default.
- W857119568 hasConcept C39961270 @default.
- W857119568 hasConcept C4746552 @default.
- W857119568 hasConcept C529278444 @default.
- W857119568 hasConcept C71924100 @default.
- W857119568 hasConcept C78519656 @default.
- W857119568 hasConcept C86803240 @default.
- W857119568 hasConcept C95444343 @default.
- W857119568 hasConceptScore W857119568C127413603 @default.
- W857119568 hasConceptScore W857119568C136834591 @default.
- W857119568 hasConceptScore W857119568C142724271 @default.
- W857119568 hasConceptScore W857119568C169760540 @default.
- W857119568 hasConceptScore W857119568C202474031 @default.
- W857119568 hasConceptScore W857119568C204232928 @default.
- W857119568 hasConceptScore W857119568C2777542384 @default.
- W857119568 hasConceptScore W857119568C2779965356 @default.
- W857119568 hasConceptScore W857119568C2780495277 @default.
- W857119568 hasConceptScore W857119568C2780496858 @default.
- W857119568 hasConceptScore W857119568C2780645631 @default.
- W857119568 hasConceptScore W857119568C28328180 @default.
- W857119568 hasConceptScore W857119568C39961270 @default.
- W857119568 hasConceptScore W857119568C4746552 @default.
- W857119568 hasConceptScore W857119568C529278444 @default.
- W857119568 hasConceptScore W857119568C71924100 @default.
- W857119568 hasConceptScore W857119568C78519656 @default.
- W857119568 hasConceptScore W857119568C86803240 @default.
- W857119568 hasConceptScore W857119568C95444343 @default.
- W857119568 hasLocation W8571195681 @default.
- W857119568 hasOpenAccess W857119568 @default.
- W857119568 hasPrimaryLocation W8571195681 @default.
- W857119568 hasRelatedWork W1176169503 @default.
- W857119568 hasRelatedWork W1815235978 @default.
- W857119568 hasRelatedWork W2003644243 @default.
- W857119568 hasRelatedWork W2009637450 @default.
- W857119568 hasRelatedWork W2071969118 @default.
- W857119568 hasRelatedWork W2098914644 @default.
- W857119568 hasRelatedWork W2100210477 @default.
- W857119568 hasRelatedWork W2114303594 @default.
- W857119568 hasRelatedWork W2126182481 @default.
- W857119568 hasRelatedWork W2151637484 @default.
- W857119568 hasRelatedWork W2302223665 @default.
- W857119568 hasRelatedWork W2483938255 @default.
- W857119568 hasRelatedWork W2513727606 @default.
- W857119568 hasRelatedWork W2735797772 @default.
- W857119568 hasRelatedWork W2738068730 @default.
- W857119568 hasRelatedWork W2942629017 @default.
- W857119568 hasRelatedWork W2980692601 @default.
- W857119568 hasRelatedWork W3029178469 @default.
- W857119568 hasRelatedWork W3173353674 @default.
- W857119568 hasRelatedWork W62519176 @default.
- W857119568 isParatext "false" @default.
- W857119568 isRetracted "false" @default.
- W857119568 magId "857119568" @default.
- W857119568 workType "dissertation" @default.