Matches in SemOpenAlex for { <https://semopenalex.org/work/W857511244> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W857511244 endingPage "545" @default.
- W857511244 startingPage "538" @default.
- W857511244 abstract "When DNA replication of simian virus 40 (SV40) is initiated on the replication origin, the regions containing the initiation sites of DNA primase, which participates in the transient RNA primer synthesis for formation of Okazaki fragments in the lagging strand, were chosen as the target sites of antisense RNA for studies of the inhibition of SV40 DNA replication. Four recombinant transcription vectors, pUC-PrI, pUC-PrII, pGEM-PrBS, and pGEM-PrSN, coding antisense RNA, were constructed. Four antisense RNAs (named as I, II, BS, and SN) having the size of 18, 19,58, and 123 nts, respectively, were made from the transcription vectors by in vitro transcription. And then, antisense RNA in the concentration of 2 were added to COS cells transfected with pATSV-W which is a recombinant plasmid containing the SV40 origin of replication. The inhibitory extent of DNA replication was measured by DpnI resistance and was confirmed by measurement of transient RNA primer synthesis. The result shows that six combinations of antisense RNA (I, II, BS, SN, I+SN, and BS+SN) lead to the inhibition of SV40 DNA replication by up to 85%." @default.
- W857511244 created "2016-06-24" @default.
- W857511244 creator A5022988530 @default.
- W857511244 creator A5041408678 @default.
- W857511244 date "1995-01-01" @default.
- W857511244 modified "2023-09-26" @default.
- W857511244 title "Effects on the Initiation of Simian Virus 40 DNA Replication by Antisense RNA" @default.
- W857511244 hasPublicationYear "1995" @default.
- W857511244 type Work @default.
- W857511244 sameAs 857511244 @default.
- W857511244 citedByCount "0" @default.
- W857511244 crossrefType "journal-article" @default.
- W857511244 hasAuthorship W857511244A5022988530 @default.
- W857511244 hasAuthorship W857511244A5041408678 @default.
- W857511244 hasConcept C104317684 @default.
- W857511244 hasConcept C104690221 @default.
- W857511244 hasConcept C11305352 @default.
- W857511244 hasConcept C138885662 @default.
- W857511244 hasConcept C143141573 @default.
- W857511244 hasConcept C147789679 @default.
- W857511244 hasConcept C153911025 @default.
- W857511244 hasConcept C156719811 @default.
- W857511244 hasConcept C179926584 @default.
- W857511244 hasConcept C185592680 @default.
- W857511244 hasConcept C188036394 @default.
- W857511244 hasConcept C194830204 @default.
- W857511244 hasConcept C202416437 @default.
- W857511244 hasConcept C41895202 @default.
- W857511244 hasConcept C54355233 @default.
- W857511244 hasConcept C552990157 @default.
- W857511244 hasConcept C58337764 @default.
- W857511244 hasConcept C67705224 @default.
- W857511244 hasConcept C73573662 @default.
- W857511244 hasConcept C7452308 @default.
- W857511244 hasConcept C86803240 @default.
- W857511244 hasConcept C98086919 @default.
- W857511244 hasConceptScore W857511244C104317684 @default.
- W857511244 hasConceptScore W857511244C104690221 @default.
- W857511244 hasConceptScore W857511244C11305352 @default.
- W857511244 hasConceptScore W857511244C138885662 @default.
- W857511244 hasConceptScore W857511244C143141573 @default.
- W857511244 hasConceptScore W857511244C147789679 @default.
- W857511244 hasConceptScore W857511244C153911025 @default.
- W857511244 hasConceptScore W857511244C156719811 @default.
- W857511244 hasConceptScore W857511244C179926584 @default.
- W857511244 hasConceptScore W857511244C185592680 @default.
- W857511244 hasConceptScore W857511244C188036394 @default.
- W857511244 hasConceptScore W857511244C194830204 @default.
- W857511244 hasConceptScore W857511244C202416437 @default.
- W857511244 hasConceptScore W857511244C41895202 @default.
- W857511244 hasConceptScore W857511244C54355233 @default.
- W857511244 hasConceptScore W857511244C552990157 @default.
- W857511244 hasConceptScore W857511244C58337764 @default.
- W857511244 hasConceptScore W857511244C67705224 @default.
- W857511244 hasConceptScore W857511244C73573662 @default.
- W857511244 hasConceptScore W857511244C7452308 @default.
- W857511244 hasConceptScore W857511244C86803240 @default.
- W857511244 hasConceptScore W857511244C98086919 @default.
- W857511244 hasIssue "6" @default.
- W857511244 hasLocation W8575112441 @default.
- W857511244 hasOpenAccess W857511244 @default.
- W857511244 hasPrimaryLocation W8575112441 @default.
- W857511244 hasRelatedWork W1530573673 @default.
- W857511244 hasRelatedWork W1551897021 @default.
- W857511244 hasRelatedWork W1563497787 @default.
- W857511244 hasRelatedWork W1856939144 @default.
- W857511244 hasRelatedWork W1927058072 @default.
- W857511244 hasRelatedWork W1970259879 @default.
- W857511244 hasRelatedWork W1971814925 @default.
- W857511244 hasRelatedWork W2015646343 @default.
- W857511244 hasRelatedWork W2039201183 @default.
- W857511244 hasRelatedWork W2042920193 @default.
- W857511244 hasRelatedWork W2053445601 @default.
- W857511244 hasRelatedWork W2076885147 @default.
- W857511244 hasRelatedWork W2080675656 @default.
- W857511244 hasRelatedWork W2095407288 @default.
- W857511244 hasRelatedWork W2111954586 @default.
- W857511244 hasRelatedWork W2112309317 @default.
- W857511244 hasRelatedWork W2123297336 @default.
- W857511244 hasRelatedWork W2157581345 @default.
- W857511244 hasRelatedWork W2170257049 @default.
- W857511244 hasRelatedWork W2246818200 @default.
- W857511244 hasVolume "28" @default.
- W857511244 isParatext "false" @default.
- W857511244 isRetracted "false" @default.
- W857511244 magId "857511244" @default.
- W857511244 workType "article" @default.