Matches in SemOpenAlex for { <https://semopenalex.org/work/W857579445> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W857579445 abstract "There is huge academic interest and clinical need associated with the development of biomarker immunoassays where general aims are the generation of highly specific, convenient and sensitive sensing formats. In this project, a powerful electrochemical technique, electrochemical impedance spectroscopy (EIS), is applied in the establishment of powerful biomarker detecting protocols. Firstly, ultrasensitive, label-free and reusable insulin sensors, based on an antibody-PEGylated thiol self-assembly monolayer (PEG thiol SAM) interface, were produced and characterised via Faradaic EIS, presenting a detection limit (LOD) of 1.2 pM, a linear range across four orders of magnitude, and high sensitivity in even 50 % serum. By applying similar surface chemistry, a label-free biosensor, specific for the detection of α-synuclein antibodies, was fabricated. The α-synuclein interfaces used enabled the reliable detecting of this biomarker in patient sample serum. The concentration levels in the control and a patient group were determined to be significantly different, and, significantly, this difference was consistently across two different cohorts. Strikingly, this could potentially underpin an entirely new means of early Parkinson’s disease (PD) diagnosis. Non-Faradaic EIS methods were additionally applied to label-free insulin assays at both PEG thiol SAM and zwitterionic polymer film interfaces. The latter presented not only an exceptionally non-fouling interface, but also one seemingly both highly biocompatible and facilitating enhanced receptor: target binding. Finally, impedance assays, though potent, generally, operate by sampling only one of a limited number of available experimental variables, typically, Rct for Faradaic EIS, or C or Z for non-Faradaic EIS. Work carried out herein also explores the generation and utility of a portfolio of mathematically derived immittance functions all obtained from the same raw data sets. A particular focus was the examination of whether these were capable of increasing assay sensitivity and efficiency above normal impedance treatments." @default.
- W857579445 created "2016-06-24" @default.
- W857579445 creator A5089776239 @default.
- W857579445 date "2013-01-01" @default.
- W857579445 modified "2023-09-27" @default.
- W857579445 title "Optimised label-free biomarker assays with electrochemical impedance spectroscopy" @default.
- W857579445 hasPublicationYear "2013" @default.
- W857579445 type Work @default.
- W857579445 sameAs 857579445 @default.
- W857579445 citedByCount "0" @default.
- W857579445 crossrefType "dissertation" @default.
- W857579445 hasAuthorship W857579445A5089776239 @default.
- W857579445 hasConcept C10138342 @default.
- W857579445 hasConcept C113196181 @default.
- W857579445 hasConcept C119128265 @default.
- W857579445 hasConcept C147789679 @default.
- W857579445 hasConcept C159654299 @default.
- W857579445 hasConcept C160756335 @default.
- W857579445 hasConcept C162324750 @default.
- W857579445 hasConcept C171250308 @default.
- W857579445 hasConcept C17525397 @default.
- W857579445 hasConcept C185592680 @default.
- W857579445 hasConcept C192562407 @default.
- W857579445 hasConcept C203014093 @default.
- W857579445 hasConcept C2780420688 @default.
- W857579445 hasConcept C2781197716 @default.
- W857579445 hasConcept C43617362 @default.
- W857579445 hasConcept C52859227 @default.
- W857579445 hasConcept C54400483 @default.
- W857579445 hasConcept C55493867 @default.
- W857579445 hasConcept C7040849 @default.
- W857579445 hasConcept C7070889 @default.
- W857579445 hasConcept C71924100 @default.
- W857579445 hasConceptScore W857579445C10138342 @default.
- W857579445 hasConceptScore W857579445C113196181 @default.
- W857579445 hasConceptScore W857579445C119128265 @default.
- W857579445 hasConceptScore W857579445C147789679 @default.
- W857579445 hasConceptScore W857579445C159654299 @default.
- W857579445 hasConceptScore W857579445C160756335 @default.
- W857579445 hasConceptScore W857579445C162324750 @default.
- W857579445 hasConceptScore W857579445C171250308 @default.
- W857579445 hasConceptScore W857579445C17525397 @default.
- W857579445 hasConceptScore W857579445C185592680 @default.
- W857579445 hasConceptScore W857579445C192562407 @default.
- W857579445 hasConceptScore W857579445C203014093 @default.
- W857579445 hasConceptScore W857579445C2780420688 @default.
- W857579445 hasConceptScore W857579445C2781197716 @default.
- W857579445 hasConceptScore W857579445C43617362 @default.
- W857579445 hasConceptScore W857579445C52859227 @default.
- W857579445 hasConceptScore W857579445C54400483 @default.
- W857579445 hasConceptScore W857579445C55493867 @default.
- W857579445 hasConceptScore W857579445C7040849 @default.
- W857579445 hasConceptScore W857579445C7070889 @default.
- W857579445 hasConceptScore W857579445C71924100 @default.
- W857579445 hasLocation W8575794451 @default.
- W857579445 hasOpenAccess W857579445 @default.
- W857579445 hasPrimaryLocation W8575794451 @default.
- W857579445 hasRelatedWork W1023380072 @default.
- W857579445 hasRelatedWork W1157903332 @default.
- W857579445 hasRelatedWork W1614786220 @default.
- W857579445 hasRelatedWork W2034673235 @default.
- W857579445 hasRelatedWork W2054660226 @default.
- W857579445 hasRelatedWork W2128740924 @default.
- W857579445 hasRelatedWork W2469716745 @default.
- W857579445 hasRelatedWork W2473362504 @default.
- W857579445 hasRelatedWork W2543433092 @default.
- W857579445 hasRelatedWork W2774694808 @default.
- W857579445 hasRelatedWork W2793766479 @default.
- W857579445 hasRelatedWork W2941045871 @default.
- W857579445 hasRelatedWork W2954222109 @default.
- W857579445 hasRelatedWork W2978435684 @default.
- W857579445 hasRelatedWork W2982003208 @default.
- W857579445 hasRelatedWork W3126431441 @default.
- W857579445 hasRelatedWork W3165877193 @default.
- W857579445 hasRelatedWork W3187943238 @default.
- W857579445 hasRelatedWork W3202741682 @default.
- W857579445 hasRelatedWork W2290708917 @default.
- W857579445 isParatext "false" @default.
- W857579445 isRetracted "false" @default.
- W857579445 magId "857579445" @default.
- W857579445 workType "dissertation" @default.