FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W857585158> ?p ?o ?g. }

• W857585158 endingPage "e0132183" @default.
• W857585158 startingPage "e0132183" @default.
• W857585158 abstract "The raphidophyte Heterosigma akashiwo is a globally distributed harmful alga that has been associated with fish kills in coastal waters. To understand the mechanisms of H. akashiwo bloom formation, gene expression analysis is often required. To accurately characterize the expression levels of a gene of interest, proper reference genes are essential. In this study, we assessed ten of the previously reported algal candidate genes (rpL17-2, rpL23, cox2, cal, tua, tub, ef1, 18S, gapdh, and mdh) for their suitability as reference genes in this species. We used qRT-PCR to quantify the expression levels of these genes in H. akashiwo grown under different temperatures, light intensities, nutrient concentrations, and time points over a diel cycle. The expression stability of these genes was evaluated using geNorm and NormFinder algorithms. Although none of these genes exhibited invariable expression levels, cal, tub, rpL17-2 and rpL23 expression levels were the most stable across the different conditions tested. For further validation, these selected genes were used to normalize the expression levels of ribulose-1, 5-bisphosphate carboxylase/oxygenase large unite (HrbcL) over a diel cycle. Results showed that the expression of HrbcL normalized against each of these reference genes was the highest at midday and lowest at midnight, similar to the diel patterns typically documented for this gene in algae. While the validated reference genes will be useful for future gene expression studies on H. akashiwo, we expect that the procedure used in this study may be helpful to future efforts to screen reference genes for other algae." @default.
• W857585158 created "2016-06-24" @default.
• W857585158 creator A5006706475 @default.
• W857585158 creator A5030528976 @default.
• W857585158 creator A5072861069 @default.
• W857585158 creator A5087201378 @default.
• W857585158 date "2015-07-02" @default.
• W857585158 modified "2023-09-30" @default.
• W857585158 title "Screening for Suitable Reference Genes for Quantitative Real-Time PCR in Heterosigma akashiwo (Raphidophyceae)" @default.
• W857585158 cites W1577577364 @default.
• W857585158 cites W1971385920 @default.
• W857585158 cites W1975652626 @default.
• W857585158 cites W1988538711 @default.
• W857585158 cites W1992504847 @default.
• W857585158 cites W2015868923 @default.
• W857585158 cites W2019619926 @default.
• W857585158 cites W2027921745 @default.
• W857585158 cites W2032317365 @default.
• W857585158 cites W2037250147 @default.
• W857585158 cites W2038484466 @default.
• W857585158 cites W2041591832 @default.
• W857585158 cites W2051405106 @default.
• W857585158 cites W2052444471 @default.
• W857585158 cites W2052766118 @default.
• W857585158 cites W2053118291 @default.
• W857585158 cites W2059197393 @default.
• W857585158 cites W2063053724 @default.
• W857585158 cites W2063872306 @default.
• W857585158 cites W2064627046 @default.
• W857585158 cites W2071794766 @default.
• W857585158 cites W2075522608 @default.
• W857585158 cites W2077960476 @default.
• W857585158 cites W2082732597 @default.
• W857585158 cites W2084676565 @default.
• W857585158 cites W2086491289 @default.
• W857585158 cites W2090157291 @default.
• W857585158 cites W2091596392 @default.
• W857585158 cites W2094439916 @default.
• W857585158 cites W2095058154 @default.
• W857585158 cites W2102634271 @default.
• W857585158 cites W2105423509 @default.
• W857585158 cites W2106492183 @default.
• W857585158 cites W2108244474 @default.
• W857585158 cites W2112982595 @default.
• W857585158 cites W2121462051 @default.
• W857585158 cites W2122749842 @default.
• W857585158 cites W2127535576 @default.
• W857585158 cites W2131712065 @default.
• W857585158 cites W2131987407 @default.
• W857585158 cites W2134877126 @default.
• W857585158 cites W2145763434 @default.
• W857585158 cites W2148633086 @default.
• W857585158 cites W2150301869 @default.
• W857585158 cites W2152038522 @default.
• W857585158 cites W2161854664 @default.
• W857585158 cites W2166642834 @default.
• W857585158 cites W2167939007 @default.
• W857585158 cites W2171353341 @default.
• W857585158 cites W2395214611 @default.
• W857585158 cites W4235460381 @default.
• W857585158 cites W4255305119 @default.
• W857585158 doi "https://doi.org/10.1371/journal.pone.0132183" @default.
• W857585158 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4489630" @default.
• W857585158 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26133173" @default.
• W857585158 hasPublicationYear "2015" @default.
• W857585158 type Work @default.
• W857585158 sameAs 857585158 @default.
• W857585158 citedByCount "23" @default.
• W857585158 countsByYear W8575851582016 @default.
• W857585158 countsByYear W8575851582017 @default.
• W857585158 countsByYear W8575851582018 @default.
• W857585158 countsByYear W8575851582019 @default.
• W857585158 countsByYear W8575851582020 @default.
• W857585158 countsByYear W8575851582021 @default.
• W857585158 countsByYear W8575851582022 @default.
• W857585158 countsByYear W8575851582023 @default.
• W857585158 crossrefType "journal-article" @default.
• W857585158 hasAuthorship W857585158A5006706475 @default.
• W857585158 hasAuthorship W857585158A5030528976 @default.
• W857585158 hasAuthorship W857585158A5072861069 @default.
• W857585158 hasAuthorship W857585158A5087201378 @default.
• W857585158 hasBestOaLocation W8575851581 @default.
• W857585158 hasConcept C104317684 @default.
• W857585158 hasConcept C120305227 @default.
• W857585158 hasConcept C142796444 @default.
• W857585158 hasConcept C150194340 @default.
• W857585158 hasConcept C18903297 @default.
• W857585158 hasConcept C2776954389 @default.
• W857585158 hasConcept C2779148672 @default.
• W857585158 hasConcept C2780892065 @default.
• W857585158 hasConcept C54355233 @default.
• W857585158 hasConcept C70721500 @default.
• W857585158 hasConcept C86803240 @default.
• W857585158 hasConceptScore W857585158C104317684 @default.
• W857585158 hasConceptScore W857585158C120305227 @default.
• W857585158 hasConceptScore W857585158C142796444 @default.
• W857585158 hasConceptScore W857585158C150194340 @default.
• W857585158 hasConceptScore W857585158C18903297 @default.

• next