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- W857892496 abstract "In the previous report (Choi et al., 1997), the angiogenin binding peptides identified from a phage-peptide library were analyzed by using the fusion proteins composed of the Escherichia coli maltose binding protein and its corresponding peptides. However, it was difficult to obtain a sufficient amount of the fusion proteins required for further analysis because of the low expression level. We now report a high level expression of the fusion protein and analysis of its anti-angiogenin activity. The use of strong T7 promoter and removal of signal sequence allowed about a 20-fold increase in the expression efficiency of the fusion protein. We were able to obtain about 10 mg of purified fusion protein from one liter of culture. The purified fusion protein showed angiogenin-specific affinity and inhibited the binding of biotinylated actin to human angiogenin at of 0.6 mM. Its anti-angiogenin activity was also revealed by the chorioallantoic membrane assay." @default.
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- W857892496 date "1998-01-01" @default.
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- W857892496 title "Expression of an Angiogenin Binding Peptide and Its Anti-Angiogenic Activity" @default.
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