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- W85845148 abstract "In this work, the layer-by-layer (LbL) technique was applied for the coating of 316L stainless steel model substrates for stents, after surface priming by functionalisation with 3-aminopropyl) triethoxysilane (APTES). APTES coatings were prepared through the deposition of an APTES acqueous-ethanol solution on the metal substrates. The morphological and physico-chemical characteristics of APTES coatings were investigated by fluorescence and atomic force microscopy (AFM), scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) analysis. APTES films consisted of globular submicrometer domains, coating the stainless steel substrates. XPS analysis demonstrated the presence of amino groups on the APTES-functionalised surface, although the preferential surface exposure of APTES alkyl chains and/or the roughness of the coatings strongly decreased the static water contact angle. Fluorescence microscopy also showed the multilayered structure of APTES coatings on stainless steel substrates. Heparin/ poly(diallyl dimethylammonium) chloride (HE/PDDA) LbL coatings were prepared on the APTESmodified surface. SEM analysis showed that surface morphological features of LbL coated samples were not homogeneous probably due to the intrinsic roughness of metal substrates. AFM phase images revealed that LbL coatings were also not homogeneous, as submicrometer domains with different features were observed. In addition, the LbL coated metal surfaces were hydrophilic with an intermediate contact angle between the ones of pure HE and PDDA: this finding again confirmed the non-homogenity of the LbL coating. Preliminary in vitro platelet adhesion tests showed that LbL coated substrates exposing HE as the last layer strongly inhibited platelet adhesion. Future efforts are in progress to increase the density of amino groups on the APTES modified coatings with the aim to promote the deposition of more homogeneous LbL coatings on model stent surfaces." @default.
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- W85845148 date "2011-06-13" @default.
- W85845148 modified "2023-09-27" @default.
- W85845148 title "Nanoscale tailoring of the surface properties of biomedical devices by layer-by-layer technique" @default.
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