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- W861437084 abstract "The total RNA was isolated from pokeweed (Phytolacca americana) leaves using the method of guanidine isothiocyanite and used as a template to amplify the deleted mutant pokeweed antiviral protein (PAP) gene by RT-PCR and then the gene was cloned into the pGEMR-T vector. The sequencing results showed that the PAP gene consisted of 711nt, which was 99.6% identical to the PAP gene reported by Lin et al (1991). The IPTG-inducible expression vector containing the PAP gene was constructed and transferred into the E. coli strain BL21 (DE3)-plysS. A specific protein was produced after induction with 0.4m mol/L IPTG and its molecular weight was 26ku. The results of the double diffusion on the agar plate and the western blotting test showed that the protein produced in E. coli was highly identical with the PAP extracted by a Frenchman from French pokeweed leaves. These revealed that PAP gene was actually achieved and exactly expressed in E. coli." @default.
- W861437084 created "2016-06-24" @default.
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- W861437084 date "2002-01-01" @default.
- W861437084 modified "2023-09-25" @default.
- W861437084 title "Cloning and Sequencing of the Pokeweed Antiviral Protein Gene and Its Expression in E.coll" @default.
- W861437084 hasPublicationYear "2002" @default.
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