Matches in SemOpenAlex for { <https://semopenalex.org/work/W863663118> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W863663118 endingPage "462" @default.
- W863663118 startingPage "454" @default.
- W863663118 abstract "Phospholipase A2 [EC 3.1.1.4] treatment of pig kidney Na+, K+-ATPase [EC 3.6.1.3] labeled with fluorescene probes at the a-chain reduced the extent of the fluorescence intensity change of an N- [p-(2-benzimidazolyl)phenyl] maleimide (BIPM) probe at Cys-964 to below one-third of the control level accompanying the accumulation of phosphoenzymes. However, it only induced a slight decrease in that of a fluorescence isothiocyanate (FITC) probe at Lys-501 with a large decrease in the rate of change. The addition of phosphatidylserine (PS) or phosphatidylinositol (PI) to the phospholipase-treated BIPM-FITC-labeled enzyme increased the rate of the FITC fluorescence change. Phospholipase treatment of the BIPM-enzyme greatly reduced the Na+, K+-ATPase activity. The addition of PS or PI to the treated enzyme induced reactivation. These data and others suggest that Cys-964 and Glu-953 (Rb+ protectable dicyclohexyl carbodiimide binding site) are located in the vicinity of the surface area of the enzyme where hydrocarbon chains of phospholipids are present, and conserved H-bonding amino acids, Thr-955 and Ser-962, are located rather near the center of a domain forming a cation binding route or cage with other hydrophobic transmembrane segments. These data may indicate that the interaction between the BIPM probe and the hydrocarbon chains of phospholipids changes in such a way as to sense the change in the binding state of various ligands accompanying the sequential appearance of reaction intermediates of the enzyme." @default.
- W863663118 created "2016-06-24" @default.
- W863663118 creator A5023455693 @default.
- W863663118 creator A5037719759 @default.
- W863663118 creator A5066732543 @default.
- W863663118 creator A5075468052 @default.
- W863663118 creator A5075555820 @default.
- W863663118 date "1994-03-01" @default.
- W863663118 modified "2023-09-27" @default.
- W863663118 title "Different Susceptibility to Phospholipase A2 Treatment of the Fluorescence Intensity Changes in the Vicinity of Cys-964 and Lys-501 in the α-Chain of Probe-Labeled Na+,K+-ATPase1" @default.
- W863663118 doi "https://doi.org/10.1093/oxfordjournals.jbchem.a124359" @default.
- W863663118 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8056757" @default.
- W863663118 hasPublicationYear "1994" @default.
- W863663118 type Work @default.
- W863663118 sameAs 863663118 @default.
- W863663118 citedByCount "5" @default.
- W863663118 crossrefType "journal-article" @default.
- W863663118 hasAuthorship W863663118A5023455693 @default.
- W863663118 hasAuthorship W863663118A5037719759 @default.
- W863663118 hasAuthorship W863663118A5066732543 @default.
- W863663118 hasAuthorship W863663118A5075468052 @default.
- W863663118 hasAuthorship W863663118A5075555820 @default.
- W863663118 hasConcept C121332964 @default.
- W863663118 hasConcept C181199279 @default.
- W863663118 hasConcept C184235292 @default.
- W863663118 hasConcept C185592680 @default.
- W863663118 hasConcept C2775997276 @default.
- W863663118 hasConcept C2776551241 @default.
- W863663118 hasConcept C2780610907 @default.
- W863663118 hasConcept C55493867 @default.
- W863663118 hasConcept C62520636 @default.
- W863663118 hasConcept C71240020 @default.
- W863663118 hasConcept C91881484 @default.
- W863663118 hasConceptScore W863663118C121332964 @default.
- W863663118 hasConceptScore W863663118C181199279 @default.
- W863663118 hasConceptScore W863663118C184235292 @default.
- W863663118 hasConceptScore W863663118C185592680 @default.
- W863663118 hasConceptScore W863663118C2775997276 @default.
- W863663118 hasConceptScore W863663118C2776551241 @default.
- W863663118 hasConceptScore W863663118C2780610907 @default.
- W863663118 hasConceptScore W863663118C55493867 @default.
- W863663118 hasConceptScore W863663118C62520636 @default.
- W863663118 hasConceptScore W863663118C71240020 @default.
- W863663118 hasConceptScore W863663118C91881484 @default.
- W863663118 hasIssue "3" @default.
- W863663118 hasLocation W8636631181 @default.
- W863663118 hasLocation W8636631182 @default.
- W863663118 hasOpenAccess W863663118 @default.
- W863663118 hasPrimaryLocation W8636631181 @default.
- W863663118 hasRelatedWork W1763215782 @default.
- W863663118 hasRelatedWork W1901294153 @default.
- W863663118 hasRelatedWork W2071259953 @default.
- W863663118 hasRelatedWork W2087236557 @default.
- W863663118 hasRelatedWork W2115477213 @default.
- W863663118 hasRelatedWork W2171787368 @default.
- W863663118 hasRelatedWork W2231553588 @default.
- W863663118 hasRelatedWork W2399081261 @default.
- W863663118 hasRelatedWork W2418073713 @default.
- W863663118 hasRelatedWork W4233500611 @default.
- W863663118 hasVolume "115" @default.
- W863663118 isParatext "false" @default.
- W863663118 isRetracted "false" @default.
- W863663118 magId "863663118" @default.
- W863663118 workType "article" @default.