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- W864014733 abstract "The liver has the unique capability to regenerate by itself after an injury. Normally adult hepatocytes maintain a perfect balance between cell gain and cell loss and they have the ability to proliferate and regenerate the organ when the hepatic tissue is damaged.Under certain circumstances like in hepatocarcinogenesis and chronic liver injury caused by drugs, viruses and toxins, when the hepatocytes proliferation is impaired, facultative hepatic stem cells called oval cells are recruited to generate the hepatic lineages of the hepatocytes and biliary cells. Hence, it was proposed that oval cells could represent a second compartment involved in the liver regeneration when the insult of the organ is too massive and proliferation of hepatocytes is suppressed.We performed the model of rat liver regeneration via oval cells using the protocol of 70% partial hepatectomy (PH) plus 2-Acetoamidofluorene (2-AAF) treatment. By histological techniques we have characterized the oval cell development, proliferation and differentiation in vivo. Oval cells are induced between 1 and 3 days after PH inside the portal field and they express the typical onco-foetal marker alpha foeto-protein (AFP), at mRNA and protein levels. Oval cells proliferate and differentiate in hepatocytes expressing albumin, and they differentiate in biliary cells expressing cytokeratin 7 (CK 7).Serial Analysis of Gene Expression (SAGE) was applied to the hepatic stem cell model of rat liver regeneration for studying gene expression pattern of liver regeneration during early stage of oval cell proliferation and differentiation. A total of 153,057 tags were analysed from normal liver (52,343 tags), from sham control treated with 2-AAF (50,502 tags), and from an early stage of the oval cell proliferation (50,212 tags). Comparative analysis of the transcriptomes from the 3 different conditions identifies 45 differentially expressed genes during oval cell regeneration, 27 up-regulated and 18 down-regulated. Temporal regulation of these genes in the context of the oval cell regeneration was studied by Real Time PCR. Up-regulated genes include: cell cycle genes like CDC42 and cyclin D1; cytoskeleton associated proteins like stathmin 1, and E-tropomodulin; signal transduction triggering genes like CDC151 and lipopolysaccharide binding protein; transporter genes like Na+/Pi co-transporter 4, phosphatidylcholine transfer protein, and ATPase H+ 34 kDa lysosomial transporter; anti-apoptotic enzyme gene like thioredoxin like 2. Kinetically down-regulated genes involved in the lipid metabolism are: hydroxyacid oxidase 3, fatty acid CoA ligase long chain 2, and steroyl CoA desaturase 1.The regulation of protein expression of the cell cycle genes CDC42 and cyclin D1 was studied by Western Blot technique, in the oval cell liver regeneration. Interestingly, cyclin D1 and CDC42 proteins are coexpressed temporally with the oval cell protein marker AFP. From the data of the present study we conclude that the cell cycle genes CDC42 and cyclin D1 could be involved in the mechanism of proliferation of the oval cell in the liver regeneration process." @default.
- W864014733 created "2016-06-24" @default.
- W864014733 creator A5014058203 @default.
- W864014733 date "2022-02-20" @default.
- W864014733 modified "2023-09-30" @default.
- W864014733 title "Serial Analysis of Gene Expression of Rat Liver Regeneration by Oval Hepatic Stem Cells" @default.
- W864014733 doi "https://doi.org/10.53846/goediss-570" @default.
- W864014733 hasPublicationYear "2022" @default.
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