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- W866330954 abstract "Objective To study the effects of interferon-alpha (TFN-α) on apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G (APOBE3G) expression by stimulating HepG2 2.2.15 cells with TEN-α, and to preliminarily investigate whether Janus kinase-signal transduction and activators of transcription (JAR-STAT) signal pathway participates in the regulation of APOBEC3G gene transcription. Methods HepG2 2.2.15 cells were treated with various concentrations of TEN-α (0, 1, 10^1, 10^2, 10^3, 10^4 U/mL) for 8 hours, or with TEN a of 10^3 U/mL for 2, 4, 6, 8, 10, 12 hours. In the above-mentioned time, cells or cultural supernatsnts were collected. The mRNA and protein expression levels of APOBEC3G and STAT 1 in HepG3 2.2.15 cells were detected by real-time fluorescent quantitation RT-PCR and Western blot respectively. The levels of HBsAg and HBeAg in the cultural supernatant of HepG2 2.2.15 cells were detected by ELISA. The levels of HBV DNA in supernatant and HBV mRNA in cells were determined by real-time PCR and RT-PCR respectively. Results The expression level of APOBEC3G was very low in HepG2 2.2.15 cells untreated with TEN-α (0 U/mL). With the rising of IFN-α concentration, AFOBEC3G mRNA and protein level rose progressively. When IFN-α concentration was 10^4 U/mL, the expression level of APOBEC3G was the highest Moreover, the expression level of STAT-I mRNA and protein also rose pro gressively, which appeared with APOBEC3G expression amount parallelly and relevantly. With the extension of time with IFN-α stimulation, APOBEC3G expression level rose obviously, which reached the highest at the S hours, and thereafter dropped gradually. When TFN-α of 10^4 U/mL stimulated 8 hours, the level of HBsAg, HBeAg, HBV DNA in cultural supernatant and the level of HBV mRNA in HepG2 2.2.15 cells were obviously lower than the cells untreated wish IFN-α. Conclusion IFN-α ran induce HepG2 2.2.15 cells to express APOBEC3G. Within she certain limits, APOBEC3G expression presents positive correlation wish IFN-α dosage and action time. The expression of APOBEC3G induced by IFN-α may be one of antiviral mechanisms of IFN-α. Whether JAK-STAT signal pathway participates in the expression of APOVEC3G induced by IFN-α need further study." @default.
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- W866330954 date "2009-05-30" @default.
- W866330954 modified "2023-09-27" @default.
- W866330954 title "干扰素－α诱导HepG2 2.2.15细胞APOBEC3G的表达及其机制" @default.
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