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- W86675970 abstract "Site-specific incorporation of unnatural amino acids into proteins in vivo can be achieved by co-expression of an orthogonal pair of suppressor tRNA and engineered aminoacyl-tRNA synthetase (ARS) that specifically ligates an unnatural amino acid to the suppressor tRNA. As a step to establish this technique, here we generated an Escherichia coli reporter strain DH10B(Tn:lacZam) by integrating amber mutated lacZ gene into the chromosome of E. coli DH10B strain. In vivo expression of E. coli amber suppressor produced blue colonies in culture plates containing X-Gal as well as dramatically increased -galactosidase activity. In addition, expression of an orthogonal pair of Saccharomyces cerevisiae suppressor and tyrosyl-tRNA synthetase also produced blue colonies as well as moderate increase of -galactosidase activity. These data demonstrate that our reporter strain will provide an efficient method to assess amber suppression in both qualitative and quantitative manners." @default.
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- W86675970 date "2009-01-01" @default.
- W86675970 modified "2023-09-23" @default.
- W86675970 title "Establishment of an In Vivo Report System for the Evaluation of Amber Suppression Activity in Escherichia coli" @default.
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