Matches in SemOpenAlex for { <https://semopenalex.org/work/W86687369> ?p ?o ?g. }
- W86687369 endingPage "16094" @default.
- W86687369 startingPage "16087" @default.
- W86687369 abstract "Human class II histocompatibility antigens were purified from the Epstein-Barr virus-transformed human B lymphoblastoid cell line LG-2 by immunoaffinity chromatography. This is the first time all three subsets have been prepared as nonradioactive materials on a milligram scale. The yields of DR, DQ, and DP from 10 g of cells were approximately 12, 2, and 0.2 mg, respectively. Cross-contamination of the subsets was found to be less than 2% when assayed by measuring the binding of antigen-specific monoclonal antibodies to antigen immobilized on fixed erythrocytes. The three purified subsets were extensively characterized. They contained no detectable invariant chain. The three proteins were distinguished by their migration on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing. The denatured antigens were susceptible to partial removal of carbohydrate by endoglycosidase H and apparently complete removal of carbohydrate by endoglycosidase F. The isolated, denatured chains differed in their affinities for radiolabeled lectins, suggesting differences in carbohydrate structures. A water-soluble form of each antigen was prepared by a controlled papain digestion of the native antigen. Both native and denatured antigens were analyzed for their reactivities with a panel of class II antigen-specific monoclonal antibodies, allowing a precise definition of the specificities of the antibodies." @default.
- W86687369 created "2016-06-24" @default.
- W86687369 creator A5010429886 @default.
- W86687369 creator A5014108185 @default.
- W86687369 creator A5040129865 @default.
- W86687369 creator A5066025994 @default.
- W86687369 creator A5071573004 @default.
- W86687369 date "1987-11-01" @default.
- W86687369 modified "2023-10-18" @default.
- W86687369 title "Purification and characterization of class II histocompatibility antigens from a homozygous human B cell line." @default.
- W86687369 cites W1483035379 @default.
- W86687369 cites W1485215137 @default.
- W86687369 cites W1493331593 @default.
- W86687369 cites W1510081009 @default.
- W86687369 cites W1510195393 @default.
- W86687369 cites W1518482258 @default.
- W86687369 cites W1526769669 @default.
- W86687369 cites W1528710182 @default.
- W86687369 cites W1533411461 @default.
- W86687369 cites W1533798391 @default.
- W86687369 cites W1534629298 @default.
- W86687369 cites W1540536783 @default.
- W86687369 cites W1569356536 @default.
- W86687369 cites W1574342568 @default.
- W86687369 cites W1583614346 @default.
- W86687369 cites W1602871146 @default.
- W86687369 cites W1775749144 @default.
- W86687369 cites W1810482515 @default.
- W86687369 cites W1893651774 @default.
- W86687369 cites W1967209308 @default.
- W86687369 cites W1973204882 @default.
- W86687369 cites W1973963051 @default.
- W86687369 cites W1975402564 @default.
- W86687369 cites W1976932647 @default.
- W86687369 cites W1980460632 @default.
- W86687369 cites W1988167843 @default.
- W86687369 cites W1989941778 @default.
- W86687369 cites W1995482121 @default.
- W86687369 cites W1999235534 @default.
- W86687369 cites W2003870859 @default.
- W86687369 cites W2007721484 @default.
- W86687369 cites W2008301793 @default.
- W86687369 cites W2010867882 @default.
- W86687369 cites W2014698430 @default.
- W86687369 cites W2022470376 @default.
- W86687369 cites W2026970642 @default.
- W86687369 cites W2031501502 @default.
- W86687369 cites W2037510535 @default.
- W86687369 cites W2039605566 @default.
- W86687369 cites W2039905044 @default.
- W86687369 cites W2045844687 @default.
- W86687369 cites W2046437067 @default.
- W86687369 cites W2055736981 @default.
- W86687369 cites W2055967995 @default.
- W86687369 cites W2057745591 @default.
- W86687369 cites W2065643911 @default.
- W86687369 cites W2072791109 @default.
- W86687369 cites W2075077207 @default.
- W86687369 cites W2081619804 @default.
- W86687369 cites W2082520164 @default.
- W86687369 cites W2083276447 @default.
- W86687369 cites W2089341748 @default.
- W86687369 cites W2099833214 @default.
- W86687369 cites W2100837269 @default.
- W86687369 cites W2101108802 @default.
- W86687369 cites W2102131116 @default.
- W86687369 cites W2108043337 @default.
- W86687369 cites W211161208 @default.
- W86687369 cites W2125568624 @default.
- W86687369 cites W2140799426 @default.
- W86687369 cites W2143282447 @default.
- W86687369 cites W2148692847 @default.
- W86687369 cites W2187834230 @default.
- W86687369 cites W2220912830 @default.
- W86687369 cites W2232494439 @default.
- W86687369 cites W2232597941 @default.
- W86687369 cites W997409578 @default.
- W86687369 cites W999208210 @default.
- W86687369 doi "https://doi.org/10.1016/s0021-9258(18)47699-5" @default.
- W86687369 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2824477" @default.
- W86687369 hasPublicationYear "1987" @default.
- W86687369 type Work @default.
- W86687369 sameAs 86687369 @default.
- W86687369 citedByCount "217" @default.
- W86687369 countsByYear W866873692012 @default.
- W86687369 countsByYear W866873692013 @default.
- W86687369 countsByYear W866873692014 @default.
- W86687369 countsByYear W866873692015 @default.
- W86687369 countsByYear W866873692017 @default.
- W86687369 countsByYear W866873692018 @default.
- W86687369 countsByYear W866873692019 @default.
- W86687369 countsByYear W866873692020 @default.
- W86687369 countsByYear W866873692021 @default.
- W86687369 countsByYear W866873692022 @default.
- W86687369 countsByYear W866873692023 @default.
- W86687369 crossrefType "journal-article" @default.
- W86687369 hasAuthorship W86687369A5010429886 @default.
- W86687369 hasAuthorship W86687369A5014108185 @default.