FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W86754994> ?p ?o ?g. }

• W86754994 abstract "ABSTRACT. Human alpha (leukocyte) interferon subtype A (HuIFN-αA) purified from extracts of transformed E. coli has the amino acid composition and sequence expected from the cDNA sequence. The molecule's disulfide bonds (Cys-1 to Cys-98 and Cys-29 to Cys-138) have been elucidated by HPLC/tryptic mapping. Spectroscopic analysis shows the molecule to have features typical of a globular protein with a densely packed, hydrophobic core. Secondary structure predictions of a high (60-70 percent) α-helix content are supported by CD and Raman spectroscopy estimates. About half of this helical content is reversibly destroyed on titration to pH 1.5. The structure predictions can be refined by other calculations and by experimental data. Ultracentrifugation and gel permeation studies show that IFN-αA undergoes a concentration- and pH-dependent reversible aggregation. Fragments of IFN-αA generated by trypsin or cyanogen bromide cleavage of the native molecule have no antiviral or receptor binding activity. Breaking of the disulfides of IFN-αA by oxidative sulfitolysis generates a labile conformational form which is immunochemically related to native IFN-αA and can regain antiviral activity by reformation of the Cys-29 to Cys-138 disulfide. Modification of the two tryptophans of IFN-αA with 2-hydroxy-5-nitrobenzyl bromide greatly reduces its antiviral, antigenic and receptor-binding activity. Enzymatic removal of the C-terminal 13 amino acids of IFN-αA has no effect on these activities. The biological activity of alpha interferon does not require the entire molecule, but is highly dependent on the tertiary structure provided by correct folding of the bulk of the molecule. The region around the critical Cys-29 to Cys-138 bond may be of special importance." @default.
• W86754994 created "2016-06-24" @default.
• W86754994 creator A5008303766 @default.
• W86754994 creator A5008997217 @default.
• W86754994 creator A5011428136 @default.
• W86754994 creator A5041359593 @default.
• W86754994 creator A5062762607 @default.
• W86754994 creator A5065786876 @default.
• W86754994 creator A5067642704 @default.
• W86754994 date "1982-01-01" @default.
• W86754994 modified "2023-10-15" @default.
• W86754994 title "STRUCTURE-FUNCTION STUDIES ON HUMAN ALPHA INTERFERON11This work was supported by Genentech, Inc. and Hoffmann-La Roche, Inc. and by grants from the National Institutes of Health to T.A.B. (AM 18677) and R.M.S. (GM 24485)." @default.
• W86754994 cites W1514823213 @default.
• W86754994 cites W1970021931 @default.
• W86754994 cites W1984547849 @default.
• W86754994 cites W1988397041 @default.
• W86754994 cites W2007054047 @default.
• W86754994 cites W2023400093 @default.
• W86754994 cites W2052242127 @default.
• W86754994 cites W2053027321 @default.
• W86754994 cites W2053418558 @default.
• W86754994 cites W2061785757 @default.
• W86754994 cites W2065301634 @default.
• W86754994 cites W2079955190 @default.
• W86754994 cites W2095091453 @default.
• W86754994 cites W2095450147 @default.
• W86754994 doi "https://doi.org/10.1016/b978-0-12-491220-5.50035-5" @default.
• W86754994 hasPublicationYear "1982" @default.
• W86754994 type Work @default.
• W86754994 sameAs 86754994 @default.
• W86754994 citedByCount "12" @default.
• W86754994 countsByYear W867549942015 @default.
• W86754994 crossrefType "book-chapter" @default.
• W86754994 hasAuthorship W86754994A5008303766 @default.
• W86754994 hasAuthorship W86754994A5008997217 @default.
• W86754994 hasAuthorship W86754994A5011428136 @default.
• W86754994 hasAuthorship W86754994A5041359593 @default.
• W86754994 hasAuthorship W86754994A5062762607 @default.
• W86754994 hasAuthorship W86754994A5065786876 @default.
• W86754994 hasAuthorship W86754994A5067642704 @default.
• W86754994 hasConcept C104317684 @default.
• W86754994 hasConcept C167625842 @default.
• W86754994 hasConcept C178790620 @default.
• W86754994 hasConcept C181199279 @default.
• W86754994 hasConcept C185592680 @default.
• W86754994 hasConcept C2776178377 @default.
• W86754994 hasConcept C2777093645 @default.
• W86754994 hasConcept C2779281246 @default.
• W86754994 hasConcept C2780340462 @default.
• W86754994 hasConcept C2909179924 @default.
• W86754994 hasConcept C32909587 @default.
• W86754994 hasConcept C515207424 @default.
• W86754994 hasConcept C54355233 @default.
• W86754994 hasConcept C55493867 @default.
• W86754994 hasConcept C71240020 @default.
• W86754994 hasConcept C75599170 @default.
• W86754994 hasConcept C86803240 @default.
• W86754994 hasConceptScore W86754994C104317684 @default.
• W86754994 hasConceptScore W86754994C167625842 @default.
• W86754994 hasConceptScore W86754994C178790620 @default.
• W86754994 hasConceptScore W86754994C181199279 @default.
• W86754994 hasConceptScore W86754994C185592680 @default.
• W86754994 hasConceptScore W86754994C2776178377 @default.
• W86754994 hasConceptScore W86754994C2777093645 @default.
• W86754994 hasConceptScore W86754994C2779281246 @default.
• W86754994 hasConceptScore W86754994C2780340462 @default.
• W86754994 hasConceptScore W86754994C2909179924 @default.
• W86754994 hasConceptScore W86754994C32909587 @default.
• W86754994 hasConceptScore W86754994C515207424 @default.
• W86754994 hasConceptScore W86754994C54355233 @default.
• W86754994 hasConceptScore W86754994C55493867 @default.
• W86754994 hasConceptScore W86754994C71240020 @default.
• W86754994 hasConceptScore W86754994C75599170 @default.
• W86754994 hasConceptScore W86754994C86803240 @default.
• W86754994 hasLocation W867549941 @default.
• W86754994 hasOpenAccess W86754994 @default.
• W86754994 hasPrimaryLocation W867549941 @default.
• W86754994 hasRelatedWork W1482624499 @default.
• W86754994 hasRelatedWork W1544937449 @default.
• W86754994 hasRelatedWork W1965359630 @default.
• W86754994 hasRelatedWork W1997868973 @default.
• W86754994 hasRelatedWork W1999259276 @default.
• W86754994 hasRelatedWork W2003004828 @default.
• W86754994 hasRelatedWork W2004114385 @default.
• W86754994 hasRelatedWork W2008060405 @default.
• W86754994 hasRelatedWork W2016986460 @default.
• W86754994 hasRelatedWork W2020089973 @default.
• W86754994 hasRelatedWork W2026413275 @default.
• W86754994 hasRelatedWork W2031747821 @default.
• W86754994 hasRelatedWork W2046434858 @default.
• W86754994 hasRelatedWork W2053170408 @default.
• W86754994 hasRelatedWork W2064952625 @default.
• W86754994 hasRelatedWork W2076427033 @default.
• W86754994 hasRelatedWork W2079395518 @default.
• W86754994 hasRelatedWork W2079955190 @default.
• W86754994 hasRelatedWork W2105692968 @default.
• W86754994 hasRelatedWork W2128445699 @default.
• W86754994 isParatext "false" @default.
• W86754994 isRetracted "false" @default.
• W86754994 magId "86754994" @default.

• next