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- W867584231 abstract "A simple and rapid homogeneous enzyme-linked binding assay for mistletoe lectin I(ML I) was developed using a coupled enzyme system of malate dehydrogenase (MDH) and D-galactose. A highly substituted MDH-galactose conjugate was prepared by employing an isothiocyanate method for formation of thiourea bond. In the presence of ML I, ML I inhibits the activity of the conjugate based on the ML I/D-galactose specific interaction. Thus, the concentration of ML I can be related to the homogeneous inhibition of the MDH-galactose conjugate. Using this method. ML I can be measured at the level of microgram per milliliter within 10 minutes." @default.
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- W867584231 date "2000-01-01" @default.
- W867584231 modified "2023-09-24" @default.
- W867584231 title "Homogeneous Enzyme-linked Binding Assay for Mistletoe Lectin I Based on the Mistletoe Lectin I/D-galactose Interaction" @default.
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