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- W869007573 abstract "Objective To characterize allergenic components of Caryota mitis pollen.Methods Extracts from Caryota mitis were prepared and analyzed by SDS-PAGE.Proteins bands were visualized by staining the gel with Coomassie blue.Antigenic properties of the separated proteins were analyzed by immuno-blotting.The best concentration of coating Caryota mitis extracts,serum dilutions and concentration of enzyme-conjugates were pretest in BSA-ELISA,which was used to detect the Caryota mitis pollen specific IgE.And the results were compared with those of skin prick test(SPT).Results For the pollen extract of Caryota mitis,more than 10 major protein bands were detected in SDS-PAGE.Three of the separated protein bands with a molecular weight of 26000Mr,12000Mr and 14000Mr showed immunoreactivity with IgE in the sera from patients with allergy to pollen of Caryota mitis.It was found the best dilution of coating pollen extract was 1:100,the proper dilution of serum was 1:5,biotin labeled antibody was 1:1000,and the concentration of strepavidin-HRP was 1:1000.Compared with SPT under this condition the coincidence rate between the results of the SPT with positive patients serum and those with BSA-ELISA was 90%.And the coincidence rate with negative patient sera was 80%.Conclusion Pollen allergens from Caryota mitis were characterized and the coincidence rate of detection between the SPA and BSA-ELISA showed high coincidence." @default.
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- W869007573 date "2009-01-01" @default.
- W869007573 modified "2023-09-28" @default.
- W869007573 title "Detection of Caryota mitis pollen specific IgE with BSA-ELISA." @default.
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