Matches in SemOpenAlex for { <https://semopenalex.org/work/W871236756> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W871236756 endingPage "90" @default.
- W871236756 startingPage "87" @default.
- W871236756 abstract "This chapter discusses the experimental study, focusing on the preparation of α- nicotinamide adenine dinucleotide phosphate (NADP+). The α-anomer of NADP+ can be readily prepared, by the direct phosphorylation of α-NAD+, and catalyzed, by Azotobacter NAD kinase. NAD kinase is prepared from Azotobacter vinelandii. The enzyme prepared has a specific activity of 9 μmol of NADP+ formed per hour per milligram protein. Into a 10-ml beaker, 77.6 mg of ATP, 1.25 ml of 20 mM α-NAD+, 1.25 ml of 0.1 M MnCl2, and 2.5 ml of water are placed. The beaker is transferred to a larger water-jacketed beaker maintained at 37° and equipped with magnetic stirring. The pH is adjusted from about 2.6 to pH 7.6, with 1 M NH4OH (approximately 0.3 ml), and the reaction is started by adding 1.5 ml of Azotobacter NAD kinase. The pH is monitored and kept near 7.6, with 10-μl aliquots of 1 M NH4OH. The results of a typical chromatographic analysis of the ultrafiltered reaction mixture are discussed in this chapter. In the procedure, the total recovery from 25 μmol of α-NAD was 15.8 μmol of α-NADP+." @default.
- W871236756 created "2016-06-24" @default.
- W871236756 creator A5069716814 @default.
- W871236756 date "1980-01-01" @default.
- W871236756 modified "2023-09-23" @default.
- W871236756 title "[12] Preparation of α-NADP+" @default.
- W871236756 cites W1494286622 @default.
- W871236756 cites W1553365848 @default.
- W871236756 cites W1591929564 @default.
- W871236756 cites W1980409558 @default.
- W871236756 cites W1984601580 @default.
- W871236756 cites W2030766455 @default.
- W871236756 cites W2402129371 @default.
- W871236756 doi "https://doi.org/10.1016/0076-6879(80)66442-8" @default.
- W871236756 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6246394" @default.
- W871236756 hasPublicationYear "1980" @default.
- W871236756 type Work @default.
- W871236756 sameAs 871236756 @default.
- W871236756 citedByCount "5" @default.
- W871236756 crossrefType "book-chapter" @default.
- W871236756 hasAuthorship W871236756A5069716814 @default.
- W871236756 hasConcept C13965031 @default.
- W871236756 hasConcept C141280058 @default.
- W871236756 hasConcept C178790620 @default.
- W871236756 hasConcept C181199279 @default.
- W871236756 hasConcept C181440489 @default.
- W871236756 hasConcept C185592680 @default.
- W871236756 hasConcept C197957613 @default.
- W871236756 hasConcept C2776159390 @default.
- W871236756 hasConcept C2776901170 @default.
- W871236756 hasConcept C2777989768 @default.
- W871236756 hasConcept C2779728014 @default.
- W871236756 hasConcept C38485361 @default.
- W871236756 hasConcept C43617362 @default.
- W871236756 hasConcept C537208039 @default.
- W871236756 hasConcept C55493867 @default.
- W871236756 hasConcept C75520062 @default.
- W871236756 hasConceptScore W871236756C13965031 @default.
- W871236756 hasConceptScore W871236756C141280058 @default.
- W871236756 hasConceptScore W871236756C178790620 @default.
- W871236756 hasConceptScore W871236756C181199279 @default.
- W871236756 hasConceptScore W871236756C181440489 @default.
- W871236756 hasConceptScore W871236756C185592680 @default.
- W871236756 hasConceptScore W871236756C197957613 @default.
- W871236756 hasConceptScore W871236756C2776159390 @default.
- W871236756 hasConceptScore W871236756C2776901170 @default.
- W871236756 hasConceptScore W871236756C2777989768 @default.
- W871236756 hasConceptScore W871236756C2779728014 @default.
- W871236756 hasConceptScore W871236756C38485361 @default.
- W871236756 hasConceptScore W871236756C43617362 @default.
- W871236756 hasConceptScore W871236756C537208039 @default.
- W871236756 hasConceptScore W871236756C55493867 @default.
- W871236756 hasConceptScore W871236756C75520062 @default.
- W871236756 hasLocation W8712367561 @default.
- W871236756 hasLocation W8712367562 @default.
- W871236756 hasOpenAccess W871236756 @default.
- W871236756 hasPrimaryLocation W8712367561 @default.
- W871236756 hasRelatedWork W1644371783 @default.
- W871236756 hasRelatedWork W1656364975 @default.
- W871236756 hasRelatedWork W1958363826 @default.
- W871236756 hasRelatedWork W1989591288 @default.
- W871236756 hasRelatedWork W2014954519 @default.
- W871236756 hasRelatedWork W2015799885 @default.
- W871236756 hasRelatedWork W2137996948 @default.
- W871236756 hasRelatedWork W2516584807 @default.
- W871236756 hasRelatedWork W2596200008 @default.
- W871236756 hasRelatedWork W3215404101 @default.
- W871236756 isParatext "false" @default.
- W871236756 isRetracted "false" @default.
- W871236756 magId "871236756" @default.
- W871236756 workType "book-chapter" @default.