Matches in SemOpenAlex for { <https://semopenalex.org/work/W87165654> ?p ?o ?g. }
Showing items 1 to 62 of
62
with 100 items per page.
- W87165654 abstract "Membrane fusion between lamellar bodies and plasma membrane is an obligatory distal step in lung surfactant secretion in alveolar type II (T2) cells. We have previously postulated that annexin A7 (A7) can facilitate such membrane fusion in vitro and can increase surfactant secretion in permeabilized T2 cells. We now report that surfactant secretagogues increase in vivo phosphorylation of A7 in T2 cells that can possibly facilitate stimulated secretion of lung surfactant. Isolated T2 cells were cultured overnight and treated with 80 nM phorbol myristate acetate (PMA) for varying periods. The cells were extracted in lysis buffer and A7 was immunoprecipitated with anti-annexin A7 antibodies that were raised against recombinant rat A7. Western blot analysis of immunoprecipitated proteins with anti- A7, anti-phosphoserine and anti-phosphothreonine antibodies showed that PMA-treatment increased A7 phosphorylation in T2 cells in a time-dependent manner at both serine and threonine residues. The phosphorylation reached maximum within 5–10 min and was elevated during the study period (30min). Treatment of T2 cells for 10 min with other secretagogues, ATP, terbutaline or calcium ionophore A23187 also showed increased phosphorylation of A7 suggesting that it was related to increased surfactant secretion. In vitro phosphorylation of recombinant A7 with a protein kinase C preparation showed increased membrane fusion activity of annexin A7. These observations suggest that secretagogue activation of protein kinase causes phosphorylation of A7 for increased membrane fusion activity to facilitate increased surfactant secretion." @default.
- W87165654 created "2016-06-24" @default.
- W87165654 creator A5007595992 @default.
- W87165654 creator A5078297250 @default.
- W87165654 creator A5085147583 @default.
- W87165654 date "2008-03-01" @default.
- W87165654 modified "2023-09-30" @default.
- W87165654 title "Surfactant Secretagogues Increase Phosphorylation of Annexin A7 in Type II Cells" @default.
- W87165654 doi "https://doi.org/10.1096/fasebj.22.1_supplement.931.1" @default.
- W87165654 hasPublicationYear "2008" @default.
- W87165654 type Work @default.
- W87165654 sameAs 87165654 @default.
- W87165654 citedByCount "0" @default.
- W87165654 crossrefType "journal-article" @default.
- W87165654 hasAuthorship W87165654A5007595992 @default.
- W87165654 hasAuthorship W87165654A5078297250 @default.
- W87165654 hasAuthorship W87165654A5085147583 @default.
- W87165654 hasConcept C104317684 @default.
- W87165654 hasConcept C11960822 @default.
- W87165654 hasConcept C153911025 @default.
- W87165654 hasConcept C185592680 @default.
- W87165654 hasConcept C195794163 @default.
- W87165654 hasConcept C2776415932 @default.
- W87165654 hasConcept C30345302 @default.
- W87165654 hasConcept C49039625 @default.
- W87165654 hasConcept C553184892 @default.
- W87165654 hasConcept C55493867 @default.
- W87165654 hasConcept C58226133 @default.
- W87165654 hasConcept C86803240 @default.
- W87165654 hasConcept C88634738 @default.
- W87165654 hasConceptScore W87165654C104317684 @default.
- W87165654 hasConceptScore W87165654C11960822 @default.
- W87165654 hasConceptScore W87165654C153911025 @default.
- W87165654 hasConceptScore W87165654C185592680 @default.
- W87165654 hasConceptScore W87165654C195794163 @default.
- W87165654 hasConceptScore W87165654C2776415932 @default.
- W87165654 hasConceptScore W87165654C30345302 @default.
- W87165654 hasConceptScore W87165654C49039625 @default.
- W87165654 hasConceptScore W87165654C553184892 @default.
- W87165654 hasConceptScore W87165654C55493867 @default.
- W87165654 hasConceptScore W87165654C58226133 @default.
- W87165654 hasConceptScore W87165654C86803240 @default.
- W87165654 hasConceptScore W87165654C88634738 @default.
- W87165654 hasIssue "S1" @default.
- W87165654 hasLocation W871656541 @default.
- W87165654 hasOpenAccess W87165654 @default.
- W87165654 hasPrimaryLocation W871656541 @default.
- W87165654 hasRelatedWork W1995588724 @default.
- W87165654 hasRelatedWork W2022133295 @default.
- W87165654 hasRelatedWork W2092383816 @default.
- W87165654 hasRelatedWork W2115604305 @default.
- W87165654 hasRelatedWork W2337603750 @default.
- W87165654 hasRelatedWork W2352423971 @default.
- W87165654 hasRelatedWork W2372813363 @default.
- W87165654 hasRelatedWork W2373168496 @default.
- W87165654 hasRelatedWork W2418730364 @default.
- W87165654 hasRelatedWork W2898046993 @default.
- W87165654 hasVolume "22" @default.
- W87165654 isParatext "false" @default.
- W87165654 isRetracted "false" @default.
- W87165654 magId "87165654" @default.
- W87165654 workType "article" @default.