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• W87168081 abstract "Focussing on interactions between tumor cells and surrounding stromal cells in tumor progression it has recently been shown that Wnt5a enhances invasiveness of low invasive MCF-7 breast cancer cells by the non-canonical signaling pathway via jun-n-terminal kinase. In this treatise, analyzing of morphologic changes through immunofluorescence staining of ß-actin in MCF-7 cells influenced by Wnt5a shows that Wnt5a leads to forced development of cell membrane evaginations and irregular cell shape in an underground independent manner. For examination of migration we used conventional wound healing assays on plastic underground with setting an artificial scratch through the cell layer on the one hand. On the other hand a new developed method is used. For this MCF-7 cells are grown on a cover slip coated with extracellular matrix (ECM) and upon subconfluence cells are transferred upside down to ECM-coated cell culture wells. That leads to complete embedding of tumor cells in ECM and thus more physiological conditions for experiment. Test results of the new migration assay show significant enhancement of tumor cell migration by Wnt5a in other proportions than in conventional wound healing assays on plastic: on plastic underground Wnt5a treated tumor cells show increased single cell migration into the wound area while the wound boarders of the control group grow together in a concentric way. On ECM migration occurs as even growing cell surface area with very few scattered single cells instead. In tests on ECM with a fixed Wnt5a source near MCF-7 cells a directional migration is observed. Treating the tumor cells with the known antagonist of canonical Wnt signaling pathway DKK-1, effects a decrease of cell membrane evaginations besides a compact cell shape on plastic underground while it leads to increased development of cell evaginations and irregular cell shape, comparable to Wnt5a on ECM underground. In migration assays on plastic underground no increase of migration is observed under influence of DKK-1, while treatin g MCF-7 with DKK-1 on ECM underground increases migratory activity of cell layer, equivalent to Wnt5a. This fact allows the conclusion that the effects of Wnt5a and DKK-1 distinguish between the underground the cells are on. Furthermore Wnt5a causes directional migration. Therefore underground can be a critical factor for tumor invasion, especially considering the partial aspect of tumor cell migration. Those observations could play a role by clearing up physiological processes in wound healing." @default.
• W87168081 created "2016-06-24" @default.
• W87168081 creator A5037794381 @default.
• W87168081 date "2022-02-20" @default.
• W87168081 modified "2023-10-14" @default.
• W87168081 title "Veränderungen der Morphologie und des Migrationsverhaltens unter dem Einfluss von Wnt5a und DKK-1 bei Brustkrebszellen" @default.
• W87168081 doi "https://doi.org/10.53846/goediss-859" @default.
• W87168081 hasPublicationYear "2022" @default.
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