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- W871809109 abstract "目的研究wt-p53基因联合自杀基因治疗恶性胶质瘤的可行性.方法体外试验以腺病毒为载体转导wt-p53和HSV-TK入C6鼠胶质瘤细胞,给以不同浓度的GCV,以MTT法检测细胞生存率.体内实验以C6鼠胶质瘤细胞和SD大鼠建立动物模型,原位注射腺病毒为载体的wt-p53和HSV-TK基因,腹腔给以GCV(15mg.kg-1day-1×14).观察荷瘤鼠生存期;强化MRI动态监测荷瘤鼠肿瘤大小;TUNEL法检测凋亡.结果wt-p53基因明显提高鼠胶质瘤细胞对HSV-TK/GCV的敏感性,p53联合HSV-TK基因转染C6的GCV ID100=0.01μg.ml-1,而单独HSV-TK转染C6的GCVID100=1μg.ml-1,约提高100倍;p53基因还可使C6细胞凋亡增加.体内p53基因联合HSV-TK/GCV治疗使荷瘤鼠生存期明显延长,凋亡明显增加(P＜0.001);强化MRI示4周肿瘤消失.结论p53基因和HSV-TK基因的联合基因治疗可作为临床上优化自杀基因治疗方案的一种可能选择." @default.
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- W871809109 date "2005-01-01" @default.
- W871809109 modified "2023-09-23" @default.
- W871809109 title "wt—p53基因联合HSV—TK／GCV对C6鼠胶质瘤杀伤作用的实验研究" @default.
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