Matches in SemOpenAlex for { <https://semopenalex.org/work/W872231095> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W872231095 abstract "Gamma-delta T cells are potent effector lymphocytes of innate immunity and are involved in anti-tumour immune surveillance. There are two major subsets of gamma-delta T cells in humans; Vdelta1 and Vdelta2 subsets. Each subset recognize tumours by separate mechanisms involving phosphoantigens and stress-induced ligands for the activating receptor NKG2D including MICA/B and UL-16 binding proteins. The Vdelta2 subset is known to recognize Multiple Myeloma (MM) but Vdelta1+ cells targeting MM has not previously been investigated. We have examined the cytotoxic potential of Vdelta1+ cells to lyse myeloma cell lines RPMI8226 and U266, and plasma cell leukaemia ARH77. Vdelta1+ cells were purified from peripheral blood mononuclear cells from healthy donors by immunomagnetic sorting and expanded (purity of 95-97%) over 3 weeks on irradiated PBMC. Vdelta1+ cells were co-cultured with target cell lines at 5:1 and 10:1 E:T ratio in a 4-hour flow cytometric killing assay. The expanded Vdelta1+ cells showed prominent anti-myeloma reactivity by specific lysis against all three cell lines, with the highest lysis of U266 cells (mean 73.23%, SD 11.9). Vdelta1+ cells isolated from peripheral blood of myeloma patient also showed high cytotoxic reactivity of 71.6% against U266 cells. Vdelta2+ gamma-delta T cell-mediated lysis was not significantly different to lysis by Vdelta1+ cells (mean 64.13%, SD 9.3). Multiple Myeloma remains incurable and most patients relapse or become resistant to conventional treatment. Our results identify myeloma-reactive Vdelta1+ gamma-delta T cells as promising candidates for a novel tumour immunotherapy." @default.
- W872231095 created "2016-06-24" @default.
- W872231095 creator A5008546894 @default.
- W872231095 creator A5041242319 @default.
- W872231095 creator A5046260764 @default.
- W872231095 date "2013-01-01" @default.
- W872231095 modified "2023-09-24" @default.
- W872231095 title "Vdelta1 gamma-delta T cell-mediated killing of myeloma cells: a new candidate for immunotherapy" @default.
- W872231095 hasPublicationYear "2013" @default.
- W872231095 type Work @default.
- W872231095 sameAs 872231095 @default.
- W872231095 citedByCount "0" @default.
- W872231095 crossrefType "journal-article" @default.
- W872231095 hasAuthorship W872231095A5008546894 @default.
- W872231095 hasAuthorship W872231095A5041242319 @default.
- W872231095 hasAuthorship W872231095A5046260764 @default.
- W872231095 hasConcept C129374314 @default.
- W872231095 hasConcept C139563560 @default.
- W872231095 hasConcept C153911025 @default.
- W872231095 hasConcept C154317977 @default.
- W872231095 hasConcept C202751555 @default.
- W872231095 hasConcept C203014093 @default.
- W872231095 hasConcept C2776090121 @default.
- W872231095 hasConcept C502942594 @default.
- W872231095 hasConcept C55493867 @default.
- W872231095 hasConcept C86803240 @default.
- W872231095 hasConcept C8891405 @default.
- W872231095 hasConceptScore W872231095C129374314 @default.
- W872231095 hasConceptScore W872231095C139563560 @default.
- W872231095 hasConceptScore W872231095C153911025 @default.
- W872231095 hasConceptScore W872231095C154317977 @default.
- W872231095 hasConceptScore W872231095C202751555 @default.
- W872231095 hasConceptScore W872231095C203014093 @default.
- W872231095 hasConceptScore W872231095C2776090121 @default.
- W872231095 hasConceptScore W872231095C502942594 @default.
- W872231095 hasConceptScore W872231095C55493867 @default.
- W872231095 hasConceptScore W872231095C86803240 @default.
- W872231095 hasConceptScore W872231095C8891405 @default.
- W872231095 hasLocation W8722310951 @default.
- W872231095 hasOpenAccess W872231095 @default.
- W872231095 hasPrimaryLocation W8722310951 @default.
- W872231095 hasRelatedWork W104239026 @default.
- W872231095 hasRelatedWork W1538068696 @default.
- W872231095 hasRelatedWork W1971712854 @default.
- W872231095 hasRelatedWork W2049506097 @default.
- W872231095 hasRelatedWork W2073756020 @default.
- W872231095 hasRelatedWork W2074969559 @default.
- W872231095 hasRelatedWork W2301553888 @default.
- W872231095 hasRelatedWork W2410127105 @default.
- W872231095 hasRelatedWork W2492209695 @default.
- W872231095 hasRelatedWork W2537399143 @default.
- W872231095 hasRelatedWork W2552219968 @default.
- W872231095 hasRelatedWork W2557707761 @default.
- W872231095 hasRelatedWork W2559487708 @default.
- W872231095 hasRelatedWork W2573592863 @default.
- W872231095 hasRelatedWork W2784571429 @default.
- W872231095 hasRelatedWork W2979483560 @default.
- W872231095 hasRelatedWork W2980463755 @default.
- W872231095 hasRelatedWork W83934021 @default.
- W872231095 hasRelatedWork W2813194789 @default.
- W872231095 hasRelatedWork W2959326152 @default.
- W872231095 isParatext "false" @default.
- W872231095 isRetracted "false" @default.
- W872231095 magId "872231095" @default.
- W872231095 workType "article" @default.