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- W873581567 abstract "建立将叠氮溴化丙锭（PMA）与定量PCR（qPCR）技术结合,用于检测热灭活菌背景条件下的大肠杆菌活菌的方法。结果表明：5min以上的强烈光照可以使PMA与DNA共价交联,同时完全钝化游离的PMA以避免＂假阴性＂结果;抑制死菌DNA扩增的最低PMA质量浓度为10μg/mL,不抑制活菌DNA扩增的最高PMA质量浓度为20μg/mL。当活菌/总菌大于1%时,经PMA预处理,可以消除热灭活菌DNA的干扰,实现对活菌的定量检测。" @default.
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- W873581567 date "2012-11-25" @default.
- W873581567 modified "2023-09-24" @default.
- W873581567 title "PMA-qPCR方法快速检测活的E.coli O157：H7" @default.
- W873581567 hasPublicationYear "2012" @default.
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