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- W874759507 abstract "The development of integrational vectors has provided a versatile system for the advance of molecular genetic and mutagenic studies in Bacillus subtilis. Over the past decade, the identification of genes of interest and the subsequent study of their structures and regulation have been greatly facilitated by the application of techniques for using integrational vectors. This chapter describes these techniques and the strategies used and vectors devised to investigate the mechanisms of gene function and regulation in B. subtilis. Based on findings, some integrational vectors carrying additional features were constructed. Several modifications have been introduced into pJHIOl in order to improve vector performance. In more recent years, a new series of integrational vectors has been developed by several laboratories. The first generally useful integrational vectors described were plasmids pHV32 and pJH10l. The presence in these plasmids of unique restriction sites allows the easy cloning of DNA fragments, and the detection of insertion can be monitored by disruption of the ampicillin or tetracycline resistance markers. The advantage of using integrational vectors is that obtaining the fusions does not necessarily result in gene disruption, and thus the pattern of regulation of essential genes can be monitored. The study of the transcriptional potential of a specific DNA fragment is best accomplished if the DNA fragment is placed in front of lacZ in a transcription-ally neutral region of the chromosome" @default.
- W874759507 created "2016-06-24" @default.
- W874759507 creator A5020986127 @default.
- W874759507 date "2014-04-30" @default.
- W874759507 modified "2023-09-25" @default.
- W874759507 title "Integrational Vectors for Genetic Manipulation in<i>Bacillus subtilis</i>" @default.
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- W874759507 doi "https://doi.org/10.1128/9781555818388.ch42" @default.
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