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- W877376264 abstract "흰느타리버섯(Pleurotus cornucopiae(Per.) Rolland)중의 단백질을 선택적으로 분리정제하기 위하여 AH-Sepharose 4B를 출발 물질로 하여 benzoyl-AH-Sepharose 4B를 합성하여 친화성 크로마토그래피하였다. 1) AH-Sepharose 4B 중의 amino기의 capacity는 겔 Iml 당 $9.5{mu}mol$ 이었으며 합성겔의 리간드인 benzoyl기의 capacity는 겔 1ml당 $9.3{mu}mol4 이었다. 2) 친화성이 있는 단백질의 총 겉보기 분자량은 255,000 돌톤 이었으며, 이는 29,500, 31,500, 34,000, 71,000 및 89,000돌톤 단백질의 복합체였다. 3) 친화성 단백질 중의 아미노산 함량은 비극성 아미노산이 45.68%, 극성 아미노산이 26.93%, 양성 아미노산이 11.81%, 그리고 음성 아미노산이15.58%였다. 4) 소수성 benzoyl기에 친화성이 있는 단백질은 비극성인 것이 선택적으로 분리되었다. 【For selective purification of protein in Pleurotus cornucopiae (Per.) Rolland, affinity chromatography was performed by benzoyl-AH-Sepharose 4B gel synthesized using AH-Sepharose 4B with starting materials. Ligand capacity of benzoyl group was 9.3 micromole per milliliter of gel. Total apparent molecular weight of affinity protein was 255KD, which were protein complex of 29.5, 31.5 34.0, 71.0 and 89.0KD, respectively. The contents of nonpolar, polar, positively charged, and negatively charged amino acid were 45.68%, 26.93%, 11.81% and 15.58%, respectively. The nonpolar protein was selectively purified by hydrophobic ligand of benzoyl group of gel.】" @default.
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- W877376264 date "1988-01-01" @default.
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- W877376264 title "Synthesis of Resin Derivatives and Purification of Protein - Synthesis of Benzoyl-AH-Sepharose 4B and Purification in Pleurotus cornucopiae(mushroom) -" @default.
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