FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W87753514> ?p ?o ?g. }

• W87753514 endingPage "75" @default.
• W87753514 startingPage "69" @default.
• W87753514 abstract "Congestive heart failure is a significant medical problem, and abnormalities in calcium handling leading to a delayed decline in the calcium transient have been identified in failing hearts. Calcium lowering during diastole in cardiac myocytes is markedly influenced by the activity of the calcium ATPase of the sarcoplasmic reticulum which pumps 2 moles of calcium per mole of ATP split from the cytosol into the vesicle of the sarcoplasmic reticulum. In patients with end-stage heart failure, decreases in the level of the SERCA pump have been identified in some reports, however, have not been found by other investigators. To determine if the SERCA2 pump could be overexpressed in the hearts of transgenic mice and what contractile alterations would result, such mice were constructed. No detrimental effects were identified in those animals and a 20% increase in SERCA pump protein lead to an increase in contractile behavior under in vivo conditions and to an accelerated calcium transient and contraction in isolated myocytes under not hemodynamically loaded conditions. Compensatory functions for a hypothyroidism-induced decrease in SERCA pump activity were identified in transgenic SERCA mice. The SERCA2 transgene lead to a functional improvement by compensating for the prolonged relaxation of papillary muscle identified in wild-type hypothyroid mice. Similarly, a compensatory effect could be identified using adenoviral vectors expressing a SERCA transgene upon infection of cardiac myocytes exposed to the phorbol ester PMA which leads to a decrease in endogenous SERCA levels. In addition to improving the calcium transient by increasing the expression of SERCA2 protein levels, diminishing the inhibitory effects of phospholamban should also lead to increased SERCA activity and corresponding improvements in calcium handling and in contractile function. To explore this in further detail a dominant negative mutant of phospholamban was constructed by changing the lysine of position 3 to glutamic acid and the arginine of position 14 to glutamic acid. Adenoviral vectors expressing antisense RNA to phospholamban were also used. Infecting neonatal and adult cardiac myocytes with dominant negative mutants of phospholamban or phospholamban antisense constructs lead to a significant increase in SERCA activity, acceleration of the calcium transient, and increased myo-cyte contractility. The decreased calcium transient occurring in end-stage congestive heart failure may therefore be improved in the future using viral vector-based increases in SERCA transgene expression or in increasing SERCA activity by using phospholamban mutants or antisense constructs." @default.
• W87753514 created "2016-06-24" @default.
• W87753514 creator A5029880524 @default.
• W87753514 date "2000-01-01" @default.
• W87753514 modified "2023-09-26" @default.
• W87753514 title "Changing the cardiac calcium transient: SERCA2 overexpression versus phospholamban inhibition" @default.
• W87753514 cites W1966104706 @default.
• W87753514 cites W1980272147 @default.
• W87753514 cites W1990204274 @default.
• W87753514 cites W2004786427 @default.
• W87753514 cites W2010576268 @default.
• W87753514 cites W2031233485 @default.
• W87753514 cites W2038813870 @default.
• W87753514 cites W2049848520 @default.
• W87753514 cites W2059769358 @default.
• W87753514 cites W2069509176 @default.
• W87753514 cites W2082398104 @default.
• W87753514 cites W2097216911 @default.
• W87753514 cites W2118237569 @default.
• W87753514 cites W2120337309 @default.
• W87753514 cites W2142754357 @default.
• W87753514 cites W2164931274 @default.
• W87753514 cites W2169732289 @default.
• W87753514 cites W2176061808 @default.
• W87753514 cites W2415909923 @default.
• W87753514 doi "https://doi.org/10.1007/978-3-642-57710-9_6" @default.
• W87753514 hasPublicationYear "2000" @default.
• W87753514 type Work @default.
• W87753514 sameAs 87753514 @default.
• W87753514 citedByCount "0" @default.
• W87753514 crossrefType "book-chapter" @default.
• W87753514 hasAuthorship W87753514A5029880524 @default.
• W87753514 hasConcept C101571972 @default.
• W87753514 hasConcept C102230213 @default.
• W87753514 hasConcept C104317684 @default.
• W87753514 hasConcept C113217602 @default.
• W87753514 hasConcept C126322002 @default.
• W87753514 hasConcept C134018914 @default.
• W87753514 hasConcept C139966638 @default.
• W87753514 hasConcept C158617107 @default.
• W87753514 hasConcept C17137333 @default.
• W87753514 hasConcept C181199279 @default.
• W87753514 hasConcept C185592680 @default.
• W87753514 hasConcept C207200792 @default.
• W87753514 hasConcept C23265538 @default.
• W87753514 hasConcept C2775953344 @default.
• W87753514 hasConcept C2778198053 @default.
• W87753514 hasConcept C2778996579 @default.
• W87753514 hasConcept C2781414619 @default.
• W87753514 hasConcept C519063684 @default.
• W87753514 hasConcept C55493867 @default.
• W87753514 hasConcept C71924100 @default.
• W87753514 hasConcept C86803240 @default.
• W87753514 hasConcept C935647 @default.
• W87753514 hasConcept C95444343 @default.
• W87753514 hasConceptScore W87753514C101571972 @default.
• W87753514 hasConceptScore W87753514C102230213 @default.
• W87753514 hasConceptScore W87753514C104317684 @default.
• W87753514 hasConceptScore W87753514C113217602 @default.
• W87753514 hasConceptScore W87753514C126322002 @default.
• W87753514 hasConceptScore W87753514C134018914 @default.
• W87753514 hasConceptScore W87753514C139966638 @default.
• W87753514 hasConceptScore W87753514C158617107 @default.
• W87753514 hasConceptScore W87753514C17137333 @default.
• W87753514 hasConceptScore W87753514C181199279 @default.
• W87753514 hasConceptScore W87753514C185592680 @default.
• W87753514 hasConceptScore W87753514C207200792 @default.
• W87753514 hasConceptScore W87753514C23265538 @default.
• W87753514 hasConceptScore W87753514C2775953344 @default.
• W87753514 hasConceptScore W87753514C2778198053 @default.
• W87753514 hasConceptScore W87753514C2778996579 @default.
• W87753514 hasConceptScore W87753514C2781414619 @default.
• W87753514 hasConceptScore W87753514C519063684 @default.
• W87753514 hasConceptScore W87753514C55493867 @default.
• W87753514 hasConceptScore W87753514C71924100 @default.
• W87753514 hasConceptScore W87753514C86803240 @default.
• W87753514 hasConceptScore W87753514C935647 @default.
• W87753514 hasConceptScore W87753514C95444343 @default.
• W87753514 hasLocation W877535141 @default.
• W87753514 hasOpenAccess W87753514 @default.
• W87753514 hasPrimaryLocation W877535141 @default.
• W87753514 hasRelatedWork W1967021616 @default.
• W87753514 hasRelatedWork W2023470676 @default.
• W87753514 hasRelatedWork W2024918253 @default.
• W87753514 hasRelatedWork W2031815368 @default.
• W87753514 hasRelatedWork W2034547248 @default.
• W87753514 hasRelatedWork W2046771956 @default.
• W87753514 hasRelatedWork W2239573166 @default.
• W87753514 hasRelatedWork W4253314918 @default.
• W87753514 hasRelatedWork W87753514 @default.
• W87753514 hasRelatedWork W89877279 @default.
• W87753514 isParatext "false" @default.
• W87753514 isRetracted "false" @default.
• W87753514 magId "87753514" @default.
• W87753514 workType "book-chapter" @default.