Matches in SemOpenAlex for { <https://semopenalex.org/work/W877540461> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W877540461 endingPage "607" @default.
- W877540461 startingPage "607" @default.
- W877540461 abstract "607 Secreted proteins (the secretome) have high potential use as sources of biomarkers of cancer. An optimized method for isolating the secreted proteome of an isogenic series of breast cancer cell lines varying from the non-tumorigenic and non-metastatic MCF10A to the metastatic MCF10CA cl. d has been developed. Cells are grown in serum free media which is harvested and partially fractionated using a tC2 SepPak cartridge. The fractions are then digested and analyzed using LC-MS/MS (peptide sequence based analyses). Repeatable protein profiles were obtained from 3 separate preparations of each cell line, and from duplicate analyses of the same line. The combined data from all fractions and analyses of each cell line were searched against the NCBI reference sequence database using a Mascot search engine. There were significant changes observed between the secreted proteomes of the cell lines. The most significant changes occurred with alpha-1-antichymotrypsin and galectin-3-binding protein which were highly secreted from the MCF10 DCIS.com and MCF10A cl. d, but undetected in MCF10A and MCF10AT cell lines. These proteins were also secreted from unrelated metastatic cell lines in our earlier research (Mbeunkui, et al. , J. Prot. Res. 2006, 5, 899-906). Other proteins showing increasing abundance in the more aggressive cell lines included alpha-1-antitrypsin, cathepsin D and quiescin Q6. The S100 proteins, associated with aggressive cancers, showed variable changes in abundance. Secretion of proteins often believed to be strictly cytosolic, such as annexin A2, was observed. These proteins are all predicted to undergo non-classical secretion (SecretomeIP, Center for Biological Sequence Analysis). The results were validated for five selected proteins by western blot analysis. The comparison of the secretome of an isogenic series of cell lines reveals proteins related to cancer aggressiveness/progression, and proteins which are potentially highly specific markers for the early detection of cancer. This research was partially supported by grants from the National Cancer Institute (5R21CA116070 to LKP) and the Susan G. Komen Breast Cancer Foundation (BCTR0402317 to LAS)." @default.
- W877540461 created "2016-06-24" @default.
- W877540461 creator A5012634036 @default.
- W877540461 creator A5049662597 @default.
- W877540461 creator A5052594464 @default.
- W877540461 creator A5086748815 @default.
- W877540461 date "2007-05-01" @default.
- W877540461 modified "2023-09-26" @default.
- W877540461 title "Profiling differentially secreted biomarkers in the MCF10 isogenic breast cancer cell lines for biomarker discovery" @default.
- W877540461 hasPublicationYear "2007" @default.
- W877540461 type Work @default.
- W877540461 sameAs 877540461 @default.
- W877540461 citedByCount "0" @default.
- W877540461 crossrefType "journal-article" @default.
- W877540461 hasAuthorship W877540461A5012634036 @default.
- W877540461 hasAuthorship W877540461A5049662597 @default.
- W877540461 hasAuthorship W877540461A5052594464 @default.
- W877540461 hasAuthorship W877540461A5086748815 @default.
- W877540461 hasConcept C104317684 @default.
- W877540461 hasConcept C104397665 @default.
- W877540461 hasConcept C121608353 @default.
- W877540461 hasConcept C153911025 @default.
- W877540461 hasConcept C156399914 @default.
- W877540461 hasConcept C173633252 @default.
- W877540461 hasConcept C181199279 @default.
- W877540461 hasConcept C2777002142 @default.
- W877540461 hasConcept C46111723 @default.
- W877540461 hasConcept C49039625 @default.
- W877540461 hasConcept C502942594 @default.
- W877540461 hasConcept C54355233 @default.
- W877540461 hasConcept C55493867 @default.
- W877540461 hasConcept C81885089 @default.
- W877540461 hasConcept C86803240 @default.
- W877540461 hasConceptScore W877540461C104317684 @default.
- W877540461 hasConceptScore W877540461C104397665 @default.
- W877540461 hasConceptScore W877540461C121608353 @default.
- W877540461 hasConceptScore W877540461C153911025 @default.
- W877540461 hasConceptScore W877540461C156399914 @default.
- W877540461 hasConceptScore W877540461C173633252 @default.
- W877540461 hasConceptScore W877540461C181199279 @default.
- W877540461 hasConceptScore W877540461C2777002142 @default.
- W877540461 hasConceptScore W877540461C46111723 @default.
- W877540461 hasConceptScore W877540461C49039625 @default.
- W877540461 hasConceptScore W877540461C502942594 @default.
- W877540461 hasConceptScore W877540461C54355233 @default.
- W877540461 hasConceptScore W877540461C55493867 @default.
- W877540461 hasConceptScore W877540461C81885089 @default.
- W877540461 hasConceptScore W877540461C86803240 @default.
- W877540461 hasLocation W8775404611 @default.
- W877540461 hasOpenAccess W877540461 @default.
- W877540461 hasPrimaryLocation W8775404611 @default.
- W877540461 hasRelatedWork W1967697284 @default.
- W877540461 hasRelatedWork W1970581409 @default.
- W877540461 hasRelatedWork W1979977780 @default.
- W877540461 hasRelatedWork W1997352005 @default.
- W877540461 hasRelatedWork W2043204071 @default.
- W877540461 hasRelatedWork W2044524126 @default.
- W877540461 hasRelatedWork W2061379637 @default.
- W877540461 hasRelatedWork W2116626275 @default.
- W877540461 hasRelatedWork W2154279182 @default.
- W877540461 hasRelatedWork W2156691430 @default.
- W877540461 hasRelatedWork W2326860745 @default.
- W877540461 hasRelatedWork W2345189580 @default.
- W877540461 hasRelatedWork W2364056684 @default.
- W877540461 hasRelatedWork W2371228589 @default.
- W877540461 hasRelatedWork W2393672160 @default.
- W877540461 hasRelatedWork W2431111568 @default.
- W877540461 hasRelatedWork W2563648585 @default.
- W877540461 hasRelatedWork W2591079377 @default.
- W877540461 hasRelatedWork W2966979556 @default.
- W877540461 hasRelatedWork W3139661469 @default.
- W877540461 hasVolume "67" @default.
- W877540461 isParatext "false" @default.
- W877540461 isRetracted "false" @default.
- W877540461 magId "877540461" @default.
- W877540461 workType "article" @default.