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- W878385919 abstract "the use of spermatozoa as vectors for introduction of exogenous dna into the oocyte during fertilization is an alternative method for generation of transgenic animals. this procedure is based on spontaneous ability of spermatozoa to bind exogenous dna. for spermatozoa to serve as appropriate vectors for exogenous dna, they should exhibit good motility and viability, because only such cells are capable of binding exogenous dna and fertilization. boar semen was subjected to transfection involving direct incubation of spermatozoa with plasmid dna, incubation with plasmid dna in the presence of dimethyl sulfoxide (dmso), and lipofectant-aided lipofection. sperm cell membrane motility and damage was evaluated before and after centrifugation and following transfection. spermatozoa motility varied according to the transfection method used. a statistically significant difference was observed between transfection methods using dmso and lipofection (p<0.05). the motility and percentage of live spermatozoa of the boar were found to decrease after centrifugation. the decrease in boar spermatozoa motility was the lowest after the direct incubation with plasmid dna and the highest after lipofection. there was no correlation between the decrease in boar spermatozoa motility and percentage of spermatozoa with damaged cell membrane. no significant effect of decreased spermatozoa motility after lipofection was found." @default.
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- W878385919 date "2010-01-01" @default.
- W878385919 modified "2023-10-01" @default.
- W878385919 title "Evaluation of the influence of three different methods of transfection on survival of boar spermatozoa." @default.
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