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- W87876711 abstract "In eukaryotes, in vivo formation of the two ribosomal subunits from four ribosomal RNAs (rRNAs) and approximately 80 ribosomal proteins (r-proteins) involves more than 150 non-ribosomal proteins and around 100 small non-coding RNAs. Ribosome biogenesis is temporally and spatially organised within three different cellular compartments: the nucleolus, nucleoplasm and cytoplasm. Despite the rising knowledge about ribosome function and structure and how ribosomal subunits assemble in vitro in bacteria, the in vivo role of many ribosomal proteins remains obscure both in pro- and eukaryotes.This work describes the systematic analysis of yeast small subunit r-proteins (rpS) in vivo function(s) in small ribosomal subunit (SSU) maturation and assembly.The results described herein demonstrate that most eukaryotic r-proteins fulfill different roles in ribosome biogenesis, making them indispensable for growth. Different r-proteins control distinct steps of nuclear and cytoplasmic pre-18S rRNA processing and, thus, ensure that only properly assembled ribosomes become engaged in translation. Furthermore several r-proteins are required for efficient nuclear export of pre-18S rRNA, suggesting that they form an interaction platform with the export machinery.In addition, in vivo analysis of rpS assembly suggests that the pre-rRNA � rpS interactions are stabilised in the course of the SSU maturation process. Finally, analysis of rpS assembly status in two mutants in which pre-SSU nuclear export is blocked (crm1, rps5) and one in which export is strongly delayed (rps15) show that key aspects of the in vivo assembly of eukaryotic r-proteins into distinct structural parts of the SSU are similar to the in vitro assembly pathway of their prokaryotic counterparts. Interestingly, the establishment of a stable assembly intermediate of the eukaryotic SSU-body, but not of the SSU-head, is closely linked to early rRNA processing events. On the other hand, formation of assembly intermediates of the head controls efficient nuclear export of the SSU and cytoplasmic pre-rRNA maturation steps.Therefore the formation of certain assembly intermediates is required to allow the rRNA processing steps or the export of the SSU to take place. The above intermediates can also contribute substantially to the quality control of the maturing subunit." @default.
- W87876711 created "2016-06-24" @default.
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- W87876711 date "2008-04-15" @default.
- W87876711 modified "2023-09-27" @default.
- W87876711 title "Analysis of the in vivo functions and assembly pathway of small subunit ribosomal proteins in Saccharomyces cerevisiae" @default.
- W87876711 hasPublicationYear "2008" @default.
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