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- W88050646 abstract "This chapter discusses a study to examine the ontogeny of mitogen responsiveness in thymus and spleen of Xenopus laevis . Lymphocyte cultures, set up in triplicate as described elsewhere in 1% fetal calf serum supplemented medium, were stimulated with either 2000 μg/ml LPS or 1 μg/ml Con A. Splenocyte responses could be checked only after metamorphosis as the larval spleen provides too few lymphocytes for individual organ analysis. Splenocyte reactivity to Con A increased gradually until 12 months of age, whereas LPS stimulation peaked at 8–9 months before persisting at a slightly lower level in older animals. The induced mitogenesis of larval thymocytes until 5–6 weeks of age was not observed when a low but significant stimulation was achieved with both Con A and LPS. This stimulation then disappeared over metamorphosis, before reemerging during early adult life. Autoradiographic studies on cytocentrifuge preparations of 3 HTdR-pulsed thymocyte cultures have revealed increases in the proportion of labeled lymphocytes from a mean of 1.05–0.7% in control cultures, to 5.35–1.5% LPS treatment, and to 11.25–2.0% in PHA-treated cultures." @default.
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- W88050646 date "1981-01-01" @default.
- W88050646 modified "2023-09-26" @default.
- W88050646 title "ONTOGENY OF MITOGEN RESPONSIVENESS IN THYMUS AND SPLEEN OF XENOPUS LAEVIS" @default.
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- W88050646 doi "https://doi.org/10.1016/b978-0-08-025922-2.50080-2" @default.
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