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- W88091312 abstract "As hyaluronic acid shows an inhibitory effect on the synthesis of proteoglycans by cultured chondrocytes and depresses the differentiation of limb bud cells into chondrocytes, it has been considered that chondrocytes with or without hyaluronic acid would present a system well suited for the analysis of the mechanisms of interaction between cells and molecules surrounding them. This chapter discusses a study in which a culture system of chondrocytes in suspension was developed and the effect of hyaluronic acid (HA) on the chondrocytes was studied. In the study, chondrocytes, obtained from sterna of 13-day-old chick embryo, were cultured following the procedure of Schiltz et al. These floated cells were collected and used for the experiments. The floated cells could be subculture every 6 days. More than 90% of the cells remained floating in suspension in subsequent cultures, synthesizing chondrocyte-specific proteoglycan at a constant rate throughout the culture period tested (up to 55 days)." @default.
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- W88091312 date "1979-01-01" @default.
- W88091312 modified "2023-09-26" @default.
- W88091312 title "The Effect of Hyaluronic Acid on the Synthesis of Proteoglycans by Chondrocytes" @default.
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- W88091312 doi "https://doi.org/10.1016/b978-0-12-301302-6.50057-9" @default.
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