Matches in SemOpenAlex for { <https://semopenalex.org/work/W880924596> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W880924596 abstract "DNA adduct is a piece of DNA covalently bond to chemicals. Adducts activate repair processes and, unless repaired prior to replication, may lead to nucleotide substitutions, deletions, and other rearrangements. As alterations of the genetic material can cause severe diseases including cancer, inflammation, and neurodegenerative disorders, tests on mutagenicity/genotoxicity have become an integral part of risk assessment during the development of any new chemical. A number of in-vitro tests using different cell lines and in vivo tests mainly using rodents are available to identify hazardous compounds. The majority of these tests are time-consuming, labor-intensive and hardly automatable. So in search for alternative methods, the usefulness of electrochemistry (EC)-liquid chromatography (LC)-mass spectrometry (MS) was evaluated. Generally, EC represents a powerful tool to study in vitro biological oxidation processes of a number of different compounds, including drugs, peptides and proteins. Here, we used EC to initiate adduct formation. The obtained reaction products were separated by LC and detected by MS. Tandem MS experiments were used for structural confirmation. In a proof of principle study acetaminophen was selected as model compound. Covalent adduct formation was observed for electrochemical activated mixtures of acetaminophen and guanosine. Mechanistic studies revealed that adduct formation will only start at electrochemical potentials sufficiently high to initiate oxidation of both acetaminophen and guanosine. The stringent necessity of coactivation sheds a new light on the mechanism of adduct formation, because according to the generally accepted theory activation of the adduct forming agent should be sufficient. Chromatographic separation enabled the differentiation of four isomeric forms. Their connection to acetaminophen was proven in dose-response experiments. EC/LC/MS represents a fast, simple, convenient and functional tool to study DNA adduct formation which has great potential to complement the existing battery of mutagenicity/genotoxicity tests." @default.
- W880924596 created "2016-06-24" @default.
- W880924596 creator A5015489808 @default.
- W880924596 creator A5015550128 @default.
- W880924596 creator A5048166435 @default.
- W880924596 creator A5053209074 @default.
- W880924596 creator A5057033806 @default.
- W880924596 creator A5064305101 @default.
- W880924596 date "2011-10-01" @default.
- W880924596 modified "2023-09-24" @default.
- W880924596 title "Electrochemical Simulation of Covalent DNA Adduct Formation Monitored with Liquid Chromatography/Mass Spectrometry" @default.
- W880924596 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3186521" @default.
- W880924596 hasPublicationYear "2011" @default.
- W880924596 type Work @default.
- W880924596 sameAs 880924596 @default.
- W880924596 citedByCount "0" @default.
- W880924596 crossrefType "journal-article" @default.
- W880924596 hasAuthorship W880924596A5015489808 @default.
- W880924596 hasAuthorship W880924596A5015550128 @default.
- W880924596 hasAuthorship W880924596A5048166435 @default.
- W880924596 hasAuthorship W880924596A5053209074 @default.
- W880924596 hasAuthorship W880924596A5057033806 @default.
- W880924596 hasAuthorship W880924596A5064305101 @default.
- W880924596 hasConcept C108204754 @default.
- W880924596 hasConcept C143425029 @default.
- W880924596 hasConcept C147789679 @default.
- W880924596 hasConcept C162356407 @default.
- W880924596 hasConcept C17525397 @default.
- W880924596 hasConcept C178790620 @default.
- W880924596 hasConcept C180577832 @default.
- W880924596 hasConcept C185592680 @default.
- W880924596 hasConcept C207001950 @default.
- W880924596 hasConcept C21951064 @default.
- W880924596 hasConcept C2777995097 @default.
- W880924596 hasConcept C2778722691 @default.
- W880924596 hasConcept C2778959862 @default.
- W880924596 hasConcept C2781247443 @default.
- W880924596 hasConcept C29730261 @default.
- W880924596 hasConcept C31827203 @default.
- W880924596 hasConcept C43617362 @default.
- W880924596 hasConcept C52859227 @default.
- W880924596 hasConcept C54355233 @default.
- W880924596 hasConcept C552990157 @default.
- W880924596 hasConcept C55493867 @default.
- W880924596 hasConcept C86803240 @default.
- W880924596 hasConceptScore W880924596C108204754 @default.
- W880924596 hasConceptScore W880924596C143425029 @default.
- W880924596 hasConceptScore W880924596C147789679 @default.
- W880924596 hasConceptScore W880924596C162356407 @default.
- W880924596 hasConceptScore W880924596C17525397 @default.
- W880924596 hasConceptScore W880924596C178790620 @default.
- W880924596 hasConceptScore W880924596C180577832 @default.
- W880924596 hasConceptScore W880924596C185592680 @default.
- W880924596 hasConceptScore W880924596C207001950 @default.
- W880924596 hasConceptScore W880924596C21951064 @default.
- W880924596 hasConceptScore W880924596C2777995097 @default.
- W880924596 hasConceptScore W880924596C2778722691 @default.
- W880924596 hasConceptScore W880924596C2778959862 @default.
- W880924596 hasConceptScore W880924596C2781247443 @default.
- W880924596 hasConceptScore W880924596C29730261 @default.
- W880924596 hasConceptScore W880924596C31827203 @default.
- W880924596 hasConceptScore W880924596C43617362 @default.
- W880924596 hasConceptScore W880924596C52859227 @default.
- W880924596 hasConceptScore W880924596C54355233 @default.
- W880924596 hasConceptScore W880924596C552990157 @default.
- W880924596 hasConceptScore W880924596C55493867 @default.
- W880924596 hasConceptScore W880924596C86803240 @default.
- W880924596 hasLocation W8809245961 @default.
- W880924596 hasOpenAccess W880924596 @default.
- W880924596 hasPrimaryLocation W8809245961 @default.
- W880924596 hasRelatedWork W1148664835 @default.
- W880924596 hasRelatedWork W1489408430 @default.
- W880924596 hasRelatedWork W1996132242 @default.
- W880924596 hasRelatedWork W1998935419 @default.
- W880924596 hasRelatedWork W2000100379 @default.
- W880924596 hasRelatedWork W2045948766 @default.
- W880924596 hasRelatedWork W2048248962 @default.
- W880924596 hasRelatedWork W2076296059 @default.
- W880924596 hasRelatedWork W2078765127 @default.
- W880924596 hasRelatedWork W2106485698 @default.
- W880924596 hasRelatedWork W2107938838 @default.
- W880924596 hasRelatedWork W2340192401 @default.
- W880924596 hasRelatedWork W2410354784 @default.
- W880924596 hasRelatedWork W2511496302 @default.
- W880924596 hasRelatedWork W2518557402 @default.
- W880924596 hasRelatedWork W2587131755 @default.
- W880924596 hasRelatedWork W3048061215 @default.
- W880924596 hasRelatedWork W3135629852 @default.
- W880924596 hasRelatedWork W58378120 @default.
- W880924596 hasRelatedWork W2345254348 @default.
- W880924596 hasVolume "22" @default.
- W880924596 isParatext "false" @default.
- W880924596 isRetracted "false" @default.
- W880924596 magId "880924596" @default.
- W880924596 workType "article" @default.