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• W881163744 abstract "The hyperthermophile archaeon, Pyrococcus furiosus encodes two eukaryotic TFIIB family proteins, TFB1 and TFB2. TFB1 is very similar to TFIIB in terms of sequence homology and function, whereas TFB2 is unusual as it is missing highly conserved sequences in its N-terminal domain that are present in TFIIB and TFB1. Despite this, TFB2 is effective in transcription process, albeit with lower efficiency compared to TFB1. Other archaea also contain multiple TFBs, but unlike Pyrococcus furiosus TFB2, these multiple TFBs have higher sequence homology to each other and have similar transcription efficiencies. Photochemical cross-linking experiments have shown that the B-reader of TFB in archaea and TFIIB in eukaryotes is close to transcription start site and is very important in RNAP recruitment to promoter DNA and transcription start site selection. Thus the lack of the highly conserved B reader region in P. furiosus TFB2 presents the opportunity to further study the functional importance of this region. In this study several amino acids in N-terminal domain of TFB2 were mutated with photoactivable unnatural amino acid p-benzoyl L- phenylalanine (pBpa) and the proximity of TFB2 relative to DNA was determined by photochemical cross-linking experiments. The results showed that TFB2 interacts with DNA near the TATA box via its C-terminal domain, and interacts with both strands of DNA near the transcription start site via its divergent B-reader and the B-linker sequences. The B-reader loop region is close to transcription start site and interacts with the transcribed strand of promoter DNA while the B-linker strand cross-links with the non-transcribed strand. Some of the amino acids in between the B-reader loop and the B-linker strand region in TFB2 are seen to cross-link both the transcribed and the non-transcribed strand. Thus, despite the absence of strong homology to conserved B-reader and B-linker sequences, TFB2 is likely to interact with DNA in the transcription bubble and facilitate in transcription initiation." @default.
• W881163744 created "2016-06-24" @default.
• W881163744 creator A5037341374 @default.
• W881163744 date "2000-01-01" @default.
• W881163744 modified "2023-09-26" @default.
• W881163744 title "The orientation of the Pyrococcus furiosus transcription factor TFB2 in the transcription initiation complex" @default.
• W881163744 cites W1489183905 @default.
• W881163744 cites W1493378561 @default.
• W881163744 cites W1496884580 @default.
• W881163744 cites W1520930575 @default.
• W881163744 cites W1549749234 @default.
• W881163744 cites W1855181403 @default.
• W881163744 cites W1968493087 @default.
• W881163744 cites W1969670694 @default.
• W881163744 cites W1972091887 @default.
• W881163744 cites W1973488942 @default.
• W881163744 cites W1976658573 @default.
• W881163744 cites W1983911140 @default.
• W881163744 cites W1984583080 @default.
• W881163744 cites W1984657015 @default.
• W881163744 cites W1988795249 @default.
• W881163744 cites W1988822278 @default.
• W881163744 cites W1993786968 @default.
• W881163744 cites W1994239781 @default.
• W881163744 cites W1994330097 @default.
• W881163744 cites W1996233200 @default.
• W881163744 cites W1998239575 @default.
• W881163744 cites W2000266493 @default.
• W881163744 cites W2001469156 @default.
• W881163744 cites W2003019297 @default.
• W881163744 cites W2003188984 @default.
• W881163744 cites W2010118412 @default.
• W881163744 cites W2012120921 @default.
• W881163744 cites W2013235095 @default.
• W881163744 cites W2015520639 @default.
• W881163744 cites W2017754509 @default.
• W881163744 cites W2020363034 @default.
• W881163744 cites W2024190472 @default.
• W881163744 cites W2033244846 @default.
• W881163744 cites W2033565485 @default.
• W881163744 cites W2035579768 @default.
• W881163744 cites W2036238731 @default.
• W881163744 cites W2037089917 @default.
• W881163744 cites W2039751676 @default.
• W881163744 cites W2039922463 @default.
• W881163744 cites W2045080033 @default.
• W881163744 cites W2047538154 @default.
• W881163744 cites W2052094781 @default.
• W881163744 cites W2053187487 @default.
• W881163744 cites W2056273035 @default.
• W881163744 cites W2056416339 @default.
• W881163744 cites W2056835334 @default.
• W881163744 cites W2058411330 @default.
• W881163744 cites W2061040019 @default.
• W881163744 cites W2070280762 @default.
• W881163744 cites W2071090620 @default.
• W881163744 cites W2072420783 @default.
• W881163744 cites W2075716499 @default.
• W881163744 cites W2076475173 @default.
• W881163744 cites W2080587896 @default.
• W881163744 cites W2082286024 @default.
• W881163744 cites W2082395806 @default.
• W881163744 cites W2084454227 @default.
• W881163744 cites W2087447141 @default.
• W881163744 cites W2088385058 @default.
• W881163744 cites W2089550934 @default.
• W881163744 cites W2093206038 @default.
• W881163744 cites W2096567462 @default.
• W881163744 cites W2098386120 @default.
• W881163744 cites W2111441093 @default.
• W881163744 cites W2113023105 @default.
• W881163744 cites W2115659144 @default.
• W881163744 cites W2121534706 @default.
• W881163744 cites W2122212389 @default.
• W881163744 cites W2123376525 @default.
• W881163744 cites W2127201083 @default.
• W881163744 cites W2128415323 @default.
• W881163744 cites W2135037886 @default.
• W881163744 cites W2140803417 @default.
• W881163744 cites W2141735006 @default.
• W881163744 cites W2144926272 @default.
• W881163744 cites W2145952681 @default.
• W881163744 cites W2146177750 @default.
• W881163744 cites W2146265443 @default.
• W881163744 cites W2154379317 @default.
• W881163744 cites W2155523268 @default.
• W881163744 cites W2157455363 @default.
• W881163744 cites W2157714710 @default.
• W881163744 cites W2159422462 @default.
• W881163744 cites W2160658820 @default.
• W881163744 cites W2162283732 @default.
• W881163744 cites W2163368178 @default.
• W881163744 cites W2164154631 @default.
• W881163744 cites W2165783841 @default.
• W881163744 cites W2166865790 @default.
• W881163744 cites W2167324750 @default.
• W881163744 cites W2181837670 @default.
• W881163744 cites W3145335483 @default.
• W881163744 cites W2417397257 @default.
• W881163744 doi "https://doi.org/10.15760/etd.1937" @default.

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