Matches in SemOpenAlex for { <https://semopenalex.org/work/W88197322> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W88197322 abstract "Background: Bifidobacteria represent an important part of humangastrointestinal tract maintaining and balancing properintestinal microflora. Their correct identification is crucialfor taxonomical, epidemiological and clinical purposes.Objectives: The aim of this work was to evaluate capabilitiesof automated ribotyping for identification of bifidobacterialisolates from human intestine. Methods: A set of 70Bifidobacterium strains originating from intestinal mucoustissue samples from children patients was identified byMALDI-TOF MS performed on an Ultraflex III instrument (BrukerDaltonik) and further characterized using automated ribotypingperformed by the RiboPrinter system (DuPont Qualicon). Results:MALDI-TOF MS analysis enabled reliable identification (BioTyperlog(score) > 2.3) of Bifidobacterium longum (36 strains),Bifidobacterium adolescentis (23), Bifidobacterium catenulatum(7), Bifidobacterium breve (3) and Bifidobacterium animalis(1). The RiboPrinter system assigned only 33 strains to thespecies level and identified B. longum (18 strains), B.adolescentis (10), B. breve (3) and single representatives ofB. animalis and Bifidobacterium boum. These outcomes werecongruent with MALDI-TOF MS results except for two B.adolescentis strains which were identified by ribotyping as B.longum and B. boum, respectively, and six B. catenulatumstrains assigned as B. adolescentis. The remaining isolateswere not identified as they did not exceed the threshold of 85% similarity with the RiboPrinter reference database entries.Conclusions: The RiboPrinter system, which identified correctlyonly 35 % of analysed strains, has been shown as an unsuitabletool for identification of Bifidobacterium spp. occurring inhuman intestines. This work was supported by projects 2B08068,MSM0021622415, MSM0021622416, and LC06034." @default.
- W88197322 created "2016-06-24" @default.
- W88197322 creator A5005736594 @default.
- W88197322 creator A5018755097 @default.
- W88197322 creator A5019949740 @default.
- W88197322 creator A5078167880 @default.
- W88197322 creator A5078380945 @default.
- W88197322 creator A5080479735 @default.
- W88197322 date "2011-01-01" @default.
- W88197322 modified "2023-10-06" @default.
- W88197322 title "Identification of Bifidobacterium spp. isolated from childrenintestinal mucous tissue samples by MALDI-TOF MS and automatedribotyping" @default.
- W88197322 hasPublicationYear "2011" @default.
- W88197322 type Work @default.
- W88197322 sameAs 88197322 @default.
- W88197322 citedByCount "0" @default.
- W88197322 crossrefType "journal-article" @default.
- W88197322 hasAuthorship W88197322A5005736594 @default.
- W88197322 hasAuthorship W88197322A5018755097 @default.
- W88197322 hasAuthorship W88197322A5019949740 @default.
- W88197322 hasAuthorship W88197322A5078167880 @default.
- W88197322 hasAuthorship W88197322A5078380945 @default.
- W88197322 hasAuthorship W88197322A5080479735 @default.
- W88197322 hasConcept C104317684 @default.
- W88197322 hasConcept C2776996202 @default.
- W88197322 hasConcept C2777368272 @default.
- W88197322 hasConcept C2777945686 @default.
- W88197322 hasConcept C2778546792 @default.
- W88197322 hasConcept C2779001512 @default.
- W88197322 hasConcept C2779707754 @default.
- W88197322 hasConcept C2780206646 @default.
- W88197322 hasConcept C2780305828 @default.
- W88197322 hasConcept C2780512811 @default.
- W88197322 hasConcept C49105822 @default.
- W88197322 hasConcept C523546767 @default.
- W88197322 hasConcept C54355233 @default.
- W88197322 hasConcept C55493867 @default.
- W88197322 hasConcept C86803240 @default.
- W88197322 hasConcept C89423630 @default.
- W88197322 hasConceptScore W88197322C104317684 @default.
- W88197322 hasConceptScore W88197322C2776996202 @default.
- W88197322 hasConceptScore W88197322C2777368272 @default.
- W88197322 hasConceptScore W88197322C2777945686 @default.
- W88197322 hasConceptScore W88197322C2778546792 @default.
- W88197322 hasConceptScore W88197322C2779001512 @default.
- W88197322 hasConceptScore W88197322C2779707754 @default.
- W88197322 hasConceptScore W88197322C2780206646 @default.
- W88197322 hasConceptScore W88197322C2780305828 @default.
- W88197322 hasConceptScore W88197322C2780512811 @default.
- W88197322 hasConceptScore W88197322C49105822 @default.
- W88197322 hasConceptScore W88197322C523546767 @default.
- W88197322 hasConceptScore W88197322C54355233 @default.
- W88197322 hasConceptScore W88197322C55493867 @default.
- W88197322 hasConceptScore W88197322C86803240 @default.
- W88197322 hasConceptScore W88197322C89423630 @default.
- W88197322 hasLocation W881973221 @default.
- W88197322 hasOpenAccess W88197322 @default.
- W88197322 hasPrimaryLocation W881973221 @default.
- W88197322 hasRelatedWork W1983116585 @default.
- W88197322 hasRelatedWork W1989716195 @default.
- W88197322 hasRelatedWork W2025012536 @default.
- W88197322 hasRelatedWork W2027244550 @default.
- W88197322 hasRelatedWork W2027825520 @default.
- W88197322 hasRelatedWork W2035135550 @default.
- W88197322 hasRelatedWork W2060980739 @default.
- W88197322 hasRelatedWork W2081148380 @default.
- W88197322 hasRelatedWork W2099974942 @default.
- W88197322 hasRelatedWork W2109170635 @default.
- W88197322 hasRelatedWork W2109678694 @default.
- W88197322 hasRelatedWork W2126826156 @default.
- W88197322 hasRelatedWork W2149476709 @default.
- W88197322 hasRelatedWork W2151477404 @default.
- W88197322 hasRelatedWork W2183088450 @default.
- W88197322 hasRelatedWork W2186050495 @default.
- W88197322 hasRelatedWork W2243542019 @default.
- W88197322 hasRelatedWork W2347352055 @default.
- W88197322 hasRelatedWork W2349265553 @default.
- W88197322 hasRelatedWork W2591803045 @default.
- W88197322 isParatext "false" @default.
- W88197322 isRetracted "false" @default.
- W88197322 magId "88197322" @default.
- W88197322 workType "article" @default.