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- W88322504 abstract "Background: Endothelial nitric oxide synthase (eNOS)-derived nitric oxide (NO) exerts important anti-atherogenic effects. High-density lipoprotein from healthy subjects (HDLHS) stimulates endothelial NO production; however, the underlying mechanisms remain to be further defined. In the present study we therefore characterized the effects of HDLHS and HDL from patients with coronary disease (HDLCAD) on cationic amino acid transporter-1 (CAT-1), the main endothelial L-arginine transporter, and endothelial uptake of L-arginine, the substrate of eNOS. Methods: HDLHS (n=15) and HDLCAD (n=20) was isolated by sequential ultracentrifugation. The effects of HDL on endothelial mRNA and protein expression of CAT-1 were determined, and cellular localization of CAT-1 was examined by confocal microscopy. NO production was measured by electron spin resonance spectroscopy. Endothelial L-arginine uptake was determined after [3H]-labeling of L-arginine. The role of CAT-1 for HDL-dependent endothelial L-arginine uptake and ..." @default.
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- W88322504 date "2010-11-23" @default.
- W88322504 modified "2023-09-26" @default.
- W88322504 title "Abstract 19862: HDL Stimulates Endothelial CAT-1 Expression and L-Arginine Uptake: A Novel Mechanism Leading to Endothelial-Protective Effects of HDL That is Profoundly Altered in Patients With Coronary Disease" @default.
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