FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W883790177> ?p ?o ?g. }

• W883790177 abstract "Although plant biomass is among the earth’s most abundant renewable resources, the high cost of converting it to usable sugars currently prevents it from being used as an effective replacement of petroleum as a feedstock for the production chemicals, power and fuels. One way of reducing costs is to improve the performance of the enzymes used to hydrolyze plant biomass. Bacteria and fungi that degrade plant biomass produce mixtures of enzymes, including endoglucanases, cellobiohydrolases and β-glucosidases, which work synergistically to hydrolyze the polymers in plant biomass to monomeric sugars. Biochemical parameters such as cell wall structure, pH and temperature can critically influence the rate and extent of plant biomass hydrolysis. Cellobiohydrolase (Cbh) types I and II release cellobiose by hydrolyzing cellulose polymers from their reducing and non-reducing ends. Here, I have made an attempt to establish benchmark cellulase hydrolysis assays using commercially available cellulase systems and the model substrate PASC. A library of fungal cellobiohydrolases were cloned and expressed using the filamentous fungus Aspergillus niger as the expression host. Activity assays were used to identify cellobiohydrolases that could be expressed as enzymatically active enzymes by A. niger. These enzymes were then subjected to biochemical characterization using enhancement studies with commercial cellulase mixtures. In addition, the biochemical properties of the characterized cellobiohydrolases were correlated with their primary sequence information. After initial screening based on levels of expression and enzyme activity, six cellobiohydrolases were selected as promising targets. Enhancement studies of commercial cellulase mixtures using four of these enzymes was done using two experimental designs. Although the properties of the different cellobiohydrolases were similar in many respects, there were significant differences in expression levels and relative activities, which may make some more attractive as candidates for cellulase system enhancement." @default.
• W883790177 created "2016-06-24" @default.
• W883790177 creator A5009698684 @default.
• W883790177 date "2014-12-24" @default.
• W883790177 modified "2023-09-24" @default.
• W883790177 title "Expression and characterization of fungal exocellulases" @default.
• W883790177 cites W1549857682 @default.
• W883790177 cites W1926036904 @default.
• W883790177 cites W1964253821 @default.
• W883790177 cites W1982867767 @default.
• W883790177 cites W2001725154 @default.
• W883790177 cites W2008571555 @default.
• W883790177 cites W2029045342 @default.
• W883790177 cites W2033630612 @default.
• W883790177 cites W2037366756 @default.
• W883790177 cites W2046017287 @default.
• W883790177 cites W2049064737 @default.
• W883790177 cites W2059107058 @default.
• W883790177 cites W2063437430 @default.
• W883790177 cites W2077672076 @default.
• W883790177 cites W2078390073 @default.
• W883790177 cites W2086890067 @default.
• W883790177 cites W2091513159 @default.
• W883790177 cites W2132982284 @default.
• W883790177 cites W2159041869 @default.
• W883790177 cites W69389361 @default.
• W883790177 hasPublicationYear "2014" @default.
• W883790177 type Work @default.
• W883790177 sameAs 883790177 @default.
• W883790177 citedByCount "0" @default.
• W883790177 crossrefType "dissertation" @default.
• W883790177 hasAuthorship W883790177A5009698684 @default.
• W883790177 hasConcept C115540264 @default.
• W883790177 hasConcept C141603559 @default.
• W883790177 hasConcept C150903083 @default.
• W883790177 hasConcept C181199279 @default.
• W883790177 hasConcept C185592680 @default.
• W883790177 hasConcept C2775960407 @default.
• W883790177 hasConcept C2779251873 @default.
• W883790177 hasConcept C2780412358 @default.
• W883790177 hasConcept C55493867 @default.
• W883790177 hasConcept C6557445 @default.
• W883790177 hasConcept C86803240 @default.
• W883790177 hasConcept C94412978 @default.
• W883790177 hasConceptScore W883790177C115540264 @default.
• W883790177 hasConceptScore W883790177C141603559 @default.
• W883790177 hasConceptScore W883790177C150903083 @default.
• W883790177 hasConceptScore W883790177C181199279 @default.
• W883790177 hasConceptScore W883790177C185592680 @default.
• W883790177 hasConceptScore W883790177C2775960407 @default.
• W883790177 hasConceptScore W883790177C2779251873 @default.
• W883790177 hasConceptScore W883790177C2780412358 @default.
• W883790177 hasConceptScore W883790177C55493867 @default.
• W883790177 hasConceptScore W883790177C6557445 @default.
• W883790177 hasConceptScore W883790177C86803240 @default.
• W883790177 hasConceptScore W883790177C94412978 @default.
• W883790177 hasLocation W8837901771 @default.
• W883790177 hasOpenAccess W883790177 @default.
• W883790177 hasPrimaryLocation W8837901771 @default.
• W883790177 hasRelatedWork W1542524368 @default.
• W883790177 hasRelatedWork W1993248690 @default.
• W883790177 hasRelatedWork W2029391779 @default.
• W883790177 hasRelatedWork W2115328049 @default.
• W883790177 hasRelatedWork W2136590172 @default.
• W883790177 hasRelatedWork W2140497508 @default.
• W883790177 hasRelatedWork W2146132094 @default.
• W883790177 hasRelatedWork W2337333034 @default.
• W883790177 hasRelatedWork W2340221958 @default.
• W883790177 hasRelatedWork W2498312392 @default.
• W883790177 hasRelatedWork W2507945873 @default.
• W883790177 hasRelatedWork W2520670193 @default.
• W883790177 hasRelatedWork W2692228295 @default.
• W883790177 hasRelatedWork W2800633198 @default.
• W883790177 hasRelatedWork W2943857287 @default.
• W883790177 hasRelatedWork W2951346340 @default.
• W883790177 hasRelatedWork W2986512730 @default.
• W883790177 hasRelatedWork W2995765737 @default.
• W883790177 hasRelatedWork W3139612995 @default.
• W883790177 hasRelatedWork W2738467265 @default.
• W883790177 isParatext "false" @default.
• W883790177 isRetracted "false" @default.
• W883790177 magId "883790177" @default.
• W883790177 workType "dissertation" @default.