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- W88403933 abstract "A severe necrosis disease was found occurring at suburban Minghang-districts,Shanghai,during summer of 2004 and developed more widely in 2005.The typical symptoms of the disease occurred at fruiting stage were top necrosis,plant stunt,fruit distortion,foliage yellowing and slight-mosaic,combined with brown necrosis strikes on stems and leaf stalks,and developed into death of the whole plant.Virus isolates were obtained by mechanical inoculation,and characterized by double stranded-RNA analysis,morphological and cytopathological alteration tests,virus purification,protein analysis,ELISA,and CP sequence analysis.The virus causing top necrosis on tomato was identified as Tomato mosaic virus(ToMV)with some mutation.A typical isolate N5 was obtained after five cycles of single-spot isolation on Nicotiana glutinosa.The results of biological characterization tests for ToMV-N5 showed that it is similar to ToMV-1 strain.A necrosis strain of ToMV was thought out as the principal virus causing necrosis disease on tomato at Shanghai." @default.
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- W88403933 date "2007-01-01" @default.
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- W88403933 title "Characterization of viruses causing necrosis disease on tomato." @default.
- W88403933 hasPublicationYear "2007" @default.
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