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- W885455235 abstract "The virulence genes,resistance to antimicrobial drugs,PFGE pattern and cytotoxicity of the Shiga toxin-producing Escherichia coli O157 isolated from foodstuffs in Fujian province were investigated in the present study,in which the virulence genes including the stx1,stx2,hly and eaeA of O157∶H7 were examined by PCR;PFGE was used to analyze the pattern of genomic DNAs;resistance to antimicrobial drugs were performed with 15 antibiotics by K-B test and cytotoxicity of the Shiga toxin-producing E.coli O157∶H7 isolates was assayed by using the Cyto Tox 96○R kit.It was found that two out of five isolates were identified as E.coli O 157∶H7 with the presence of the virulence genes stx2,hly and eaeA,but without stx1 gene.In another 3 strains of isolates,the O157 genes were present and no H7 stx1,stx2,hly or eaeA genes could be detected.The PFGE patterns of these 5 strains of isolates were quite different,in which the homology between 2 strains approached up to 81%.The drug-susceptibility of these isolates to 15 commonly used antibiotics was high and the rates of sensitivity were 60-100%,Cytotoxicity to vero cells could be demonstrated in 2 strains of the Shiga toxin-producing isolates in a effector/target cell ratio of 50∶1 with the percentages of cytotoxicity of 61.25% and 67.8% respectively,It is concluded that there exists the Shiga toxin-producing strains of E.coli O157∶H7 with the virulence gene stx2 isolated from foodstuffs in some regions of Fujian province,indicating that the transmission of these bacteria in humans may occur and surveillance on the infection with E.coli O157∶H7 should be considered seriously." @default.
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- W885455235 date "2009-01-01" @default.
- W885455235 modified "2023-09-26" @default.
- W885455235 title "Characterization of Escherichia coli O157 isolated from foodstuffs in Fujian province." @default.
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