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- W886171330 abstract "目的：构建pp GalNacT-10基因真核表达载体。方法：采用PCR方法合成含有酶切位点（XbaⅠ、EcoRⅠ）的ppGalNacT-10cDNA。构建pMD19T-GalNacT-10-ORF和pMD19T-GalNacT-10-antisense,鉴定及测序证实cD-NA片段大小和序列。双酶切目的载体pcDNA3.1后,与GalNacT-10-ORF和GalNacT-10-antisense片段进行连接,构建正义和反义真核表达载体pcDNA3.1-GalNacT-10-ORF和pcDNA3.1-GalNacT-10-antisense,将其分别转染293T细胞,Westernblot法检测ppGalNacT-10蛋白的表达。结果：pcDNA3.1-GalNacT-10-ORF和pcDNA3.1-GalNacT-10-an-tisense包含大小、序列正确的pp GalNacT-10片段;ppGalNacT-10蛋白在转染pcDNA3.1-GalNacT-10-ORF的293T细胞中高表达,在转染pcDNA3.1-GalNacT-10-antisense的293T细胞中表达降低。结论：成功构建了ppGalNacT-10基因正义和反义真核表达载体。" @default.
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- W886171330 date "2012-01-01" @default.
- W886171330 modified "2023-09-24" @default.
- W886171330 title "pp GalNacT-10基因真核表达载体的构建及表达" @default.
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